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Anti-Mouse H-2Kb DyLight® 550

(MHC Class I)
Monoclonal Antibody

Prod. No.:H1674
Isotype:Mouse IgG2a
Conc.:0.1 mg/ml
Pkg. Size:50 µg, 100 µg, 200 µg
Starting at:$95.00
Availability:In Stock
Storage:2-8°C Detailed storage instructions below.
Mouse Anti-Mouse H-2Kb (Clone AF6- recognizes an epitope on Mouse H-2Kb. This monoclonal antibody was purified using multi-step affinity chromatography methods such as Protein A or G depending on the species and isotype.

Antigen Distribution:
The AF6-88.5 antibody reacts with H-2Kb MHC class I alloantigen expressed on nucleated cells from mice of the H-2Kb haplotype. H-2Kb is involved in antigen presentation to T cells expressing CD3/TCR and CD8 proteins. The AF6-88.5 antibody does not cross-react with other haplotypes (d, f, j, k, p, q, r, s, u, v).

Reporter Molecule:
DyLight® 550 conjugated antibodies absorb light maximally around 562 (± 4 nm) and fluoresce with a peak around 576 nm. They are brighter than TRITC conjugates, and they are equal in brightness to Cy3 and Alexa Fluor 555. All Dylight® 550 antibodies are recommended for maximum sensitivity in all immunofluorescence detection procedures within the orange-red portion of the visible spectrum. DyLight® is a trademark of Thermo Fisher Scientific Inc. and its subsidiaries.

Host Species
Mouse C57BL Splenocytes
This DyLight® 550 conjugate is formulated in 0.01 M phosphate buffered saline (PBS) pH 7.4, 150 mM NaCl, 1% BSA and 0.09% sodium azide as a preservative.
Storage and Stability
This DyLight® 550 conjugate is stable when stored at 2-8°C. Do not freeze.
Reported Applications
Each investigator should determine their own optimal working dilution for specific applications.

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