Product Datasheet
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Anti-Human HLA-A, B, C DyLight® 550

Monoclonal Antibody


Prod. No.:H1647
Clone:W6/32
Isotype:Mouse IgG2a
Conc.:0.1 mg/ml
Pkg. Size:50 µg, 100 µg, 200 µg
Storage:2-8°C Detailed storage instructions below.
Description
Specificity:
Mouse Anti-Human HLA-A, B, C (Clone W6/32) recognizes an epitope on Human HLA-A, B, C. This monoclonal antibody was purified using multi-step affinity chromatography methods such as Protein A or G depending on the species and isotype.

Antigen Distribution:
This HLA-A, B, C antigen is present on most nucleated cells of humans and old world monkeys.

Reporter Molecule:
DyLight® 550 conjugated antibodies absorb light maximally around 562 (± 4 nm) and fluoresce with a peak around 576 nm. They are brighter than TRITC conjugates, and they are equal in brightness to Cy3 and Alexa Fluor 555. All Dylight® 550 antibodies are recommended for maximum sensitivity in all immunofluorescence detection procedures within the orange-red portion of the visible spectrum. DyLight® is a trademark of Thermo Fisher Scientific Inc. and its subsidiaries.

Known Species Cross Reactivity: Hu+
Host Species
Mouse
Immunogen
Purified Recombinant Human HLA-A, B, C (>98%)
Formulation
This DyLight® 550 conjugate is formulated in 0.01 M phosphate buffered saline (PBS) pH 7.4, 150 mM NaCl, 1% BSA and 0.09% sodium azide as a preservative.
Storage and Stability
This DyLight® 550 conjugate is stable when stored at 2-8°C. Do not freeze.
Reported Applications
FC
Each investigator should determine their own optimal working dilution for specific applications.


Country of Origin
USA
References
1. Barnstable, C. et al. (1978) Cell 16:9
2. Brodsky, F. et al. (1979) Immunol. Rev. 47:3
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