Product Datasheet
www.leinco.com
excellence in early discovery research       



Anti-Human HLA-DP Monomorphic (HLA-DP) DyLight® 488

Monoclonal Antibody


Prod. No.:H1582
Clone:B7/21
Isotype:Mouse IgG3
Conc.:0.1 mg/ml
Pkg. Size:50 µg, 100 µg, 200 µg
Storage:2-8°C Detailed storage instructions below.
Description
Specificity:
Mouse Anti-Human HLA-DP Monomorphic (HLA-DP) (Clone B7/21) recognizes an epitope on Human HLA-DP. This monoclonal antibody was purified using multi-step affinity chromatography methods such as Protein A or G depending on the species and isotype.

Antigen Distribution:
The HLA-DP antigen is present on approximately 10% of normal peripheral blood lymphocytes but not on resting peripheral T-lymphocytes. HLA-DP is present in low density on peripheral blood monocytes and mitogen-stimulated T-lymphoblasts. Anti-HLA-DP reacts with most B-cell lines.

Reporter Molecule:
DyLight® 488 conjugated antibodies absorb light maximally around 493 nm (± 4 nm) and fluoresce with a peak around 518 nm. They are brighter than Cy2 and FITC conjugates and similar in brightness to Alexa Fluor 488 conjugates, but typically give less background. DyLight® 488 conjugated to any antibody is recommended to maximize sensitivity for all immunofluorescence procedures requiring a green-fluorescing dye. DyLight® is a trademark of Thermo Fisher Scientific Inc. and its subsidiaries.

Known Species Cross Reactivity: Hu+
Host Species
Mouse
Immunogen
Purified Recombinant Human HLA-DP (>98%)
Formulation
This DyLight® 488 conjugate is formulated in 0.01 M phosphate buffered saline (PBS) pH 7.4, 150 mM NaCl, 1% BSA and 0.09% sodium azide as a preservative.
Storage and Stability
This DyLight® 488 conjugate is stable when stored at 2-8°C. Do not freeze.
Reported Applications
FC
Each investigator should determine their own optimal working dilution for specific applications.


Country of Origin
USA
References
1. Watson, J. A. et al. (1983) Nature 304:358
Related Products
Anti-Human HLA-DP Monomorphic Purified Whole Molecule (Leinco Prod. No.: H127)
Anti-Human HLA-DP Monomorphic-R-phycoerythrin (Leinco Prod. No.: H130)
Anti-Human HLA-DP Monomorphic-Allophycocyanin (Leinco Prod. No.: H240)
Anti-Human HLA-DP Monomorphic-Biotin (Leinco Prod. No.: H128)
PN:
Anti-Human HLA-DP Monomorphic Purified - No Carrier Protein (Leinco Prod. No.: H260)
Anti-Human HLA-DP Monomorphic-Fluorescein (Leinco Prod. No.: H129)
800.538.1145 ® Copyright Leinco Technologies, Inc. 2006 – 2017