Product Datasheet
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Anti-Human IgG Fc Specific DyLight® 488

Monoclonal Antibody


Prod. No.:I-996
Clone:HP6043
Isotype:Mouse IgG2b
Conc.:0.1 mg/ml
Pkg. Size:50 µg, 100 µg, 200 µg
Starting at:$85.00
Availability:In Stock
Storage:2-8°C Detailed storage instructions below.
Description
Specificity:
Mouse Anti-Human IgG Fc Specific (Clone HP6043) recognizes an epitope on Human IgG Fc Specific. This monoclonal antibody was purified using multi-step affinity chromatography methods such as Protein A or G depending on the species and isotype.

Antigen Distribution:
Surface IgG is expressed on approximately 2% of normal human peripheral blood cells.

Reporter Molecule:
DyLight® 488 conjugated antibodies absorb light maximally around 493 nm (± 4 nm) and fluoresce with a peak around 518 nm. They are brighter than Cy2 and FITC conjugates and similar in brightness to Alexa Fluor 488 conjugates, but typically give less background. DyLight® 488 conjugated to any antibody is recommended to maximize sensitivity for all immunofluorescence procedures requiring a green-fluorescing dye. DyLight® is a trademark of Thermo Fisher Scientific Inc. and its subsidiaries.

Known Species Cross Reactivity: Hu+
Host Species
Mouse
Immunogen
Purified Recombinant Human IgG Fc Specific (>98%)
Formulation
This DyLight® 488 conjugate is formulated in 0.01 M phosphate buffered saline (PBS) pH 7.4, 150 mM NaCl, 1% BSA and 0.09% sodium azide as a preservative.
Storage and Stability
This DyLight® 488 conjugate is stable when stored at 2-8°C. Do not freeze.
Reported Applications
FC
Each investigator should determine their own optimal working dilution for specific applications.
Country of Origin
USA
References
1. Reimer, C. B. et al. (1984) Hybridoma 3:263
2. Jefferies, R. et al. (1985) Immunol. Letters 10:223
3. Phillips, D. J. et al. (1987) Immunol. Letters 17:159
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