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Anti-Rat PCNA (PCNA) R-phycoerythrin (R-PE)

Monoclonal Antibody


Prod. No.:P106
Clone:PC10
Isotype:Mouse IgG2a κ
Conc.:0.1 mg/ml
Pkg. Size:50 µg, 100 µg, 200 µg
Starting at:$90.00
Availability:In Stock
Storage:2-8°C Detailed storage instructions below.
Description
Specificity:
Anti-PCNA (Proliferating Cell Nuclear Antigen) recognizes polymerase delta accessory protein (Mr 36 kDa).1,3 This auxiliary protein is also known as cyclin and experiments strongly suggest that PCNA is essential for cellular DNA synthesis.2,3

Antigen Distribution:
Anti-PCNA staining is almost entirely confined to the nucleus and may show a diffuse or granular pattern or a mixture of both. Dominant PCNA expression begins during late G1 phase and is present through S phase of the cell cycle.

Reporter Molecule:
R-phycoerythrin (R-PE) has a molecular weight of 240 kDa and is purified from red algae. R-PE is an intensely bright phycobiliprotein with an absorption maxima at approximately 496, 546 and 565 nm, and a fluorescence emission maximum of ~578 nm.

Known Species Cross Reactivity: Ms+, Hu+, Rt+, All vertebrates and invertebrates
Host Species
Mouse
Immunogen
Rat PCNA made in the protein A vector pR1T2T
Formulation
This R-phycoerythrin (R-PE) conjugate is formulated in 0.01 M phosphate buffered saline (PBS) pH 7.4, 150 mM NaCl, 1% BSA and 0.09% sodium azide as a preservative.
Storage and Stability
This R-PE conjugate is stable when stored at 2-8°C. Do not freeze.
Reported Applications
FC
Flow Cytometry: This antibody can be used at a concentration of ≤ 0.125 µg per 100 µl of whole blood (or per 1.0 x 106 cells in a 100 µl total staining volume).
Each investigator should determine their own optimal working dilution for specific applications.


Country of Origin
USA
References
1. Tan, C. et al. (1987) Nucleic Acids Research 15:9299
2. Zuber, M. et al. (1989) Molec. Cell. Biol. 9:57
3. Waseem, H. N. et al. (1990) J. Cell Sci. 96:121
Related Products
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Anti-PCNA Allophycocyanin (Leinco Prod. No.: P120)
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