Product Datasheet
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Anti-Rat PCNA DyLight® 550

Monoclonal Antibody


Prod. No.:P331
Clone:PC10
Isotype:Mouse IgG2a κ
Conc.:0.1 mg/ml
Pkg. Size:50 µg, 100 µg, 200 µg
Starting at:$85.00
Availability:In Stock
Storage:2-8°C Detailed storage instructions below.
Description
Specificity:
Mouse Anti-Rat PCNA (Clone PC10) recognizes an epitope on Rat PCNA. This monoclonal antibody was purified using multi-step affinity chromatography methods such as Protein A or G depending on the species and isotype.

Antigen Distribution:
Anti-PCNA staining is almost entirely confined to the nucleus and may show a diffuse or granular pattern or a mixture of both. Dominant PCNA expression begins during late G1 phase and is present through S phase of the cell cycle.

Reporter Molecule:
DyLight® 550 conjugated antibodies absorb light maximally around 562 (± 4 nm) and fluoresce with a peak around 576 nm. They are brighter than TRITC conjugates, and they are equal in brightness to Cy3 and Alexa Fluor 555. All Dylight® 550 antibodies are recommended for maximum sensitivity in all immunofluorescence detection procedures within the orange-red portion of the visible spectrum. DyLight® is a trademark of Thermo Fisher Scientific Inc. and its subsidiaries.

Known Species Cross Reactivity: Rt+
Host Species
Mouse
Immunogen
Purified Recombinant Rat PCNA (>98%)
Formulation
This DyLight® 550 conjugate is formulated in 0.01 M phosphate buffered saline (PBS) pH 7.4, 150 mM NaCl, 1% BSA and 0.09% sodium azide as a preservative.
Storage and Stability
This DyLight® 550 conjugate is stable when stored at 2-8°C. Do not freeze.
Reported Applications
FC
Each investigator should determine their own optimal working dilution for specific applications.


Country of Origin
USA
References
1. Tan, C. et al. (1987) Nucleic Acids Research 15:9299
2. Zuber, M. et al. (1989) Molec. Cell. Biol. 9:57
3. Waseem, H. N. et al. (1990) J. Cell Sci. 96:121
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