Product Datasheet
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Anti-Rat PCNA DyLight® 594

Monoclonal Antibody


Prod. No.:P332
Clone:PC10
Isotype:Mouse IgG2a κ
Conc.:0.1 mg/ml
Pkg. Size:50 µg, 100 µg, 200 µg
Starting at:$85.00
Availability:In Stock
Storage:2-8°C Detailed storage instructions below.
Description
Specificity:
Mouse Anti-Rat PCNA (Clone PC10) recognizes an epitope on Rat PCNA. This monoclonal antibody was purified using multi-step affinity chromatography methods such as Protein A or G depending on the species and isotype.

Antigen Distribution:
Anti-PCNA staining is almost entirely confined to the nucleus and may show a diffuse or granular pattern or a mixture of both. Dominant PCNA expression begins during late G1 phase and is present through S phase of the cell cycle.

Reporter Molecule:
DyLight® 594 antibody conjugates absorb light maximally around 593 nm (± 4 nm) and fluoresce with a peak around 618 nm. They are distinctly brighter than Alexa 594 conjugates, and much brighter and more water soluble than Texas Red conjugates. DyLight® is a trademark of Thermo Fisher Scientific Inc. and its subsidiaries.

Known Species Cross Reactivity: Rt+
Host Species
Mouse
Immunogen
Purified Recombinant Rat PCNA (>98%)
Formulation
This DyLight® 594 conjugate is formulated in 0.01 M phosphate buffered saline (PBS) pH 7.4, 150 mM NaCl, 1% BSA and 0.09% sodium azide as a preservative.
Storage and Stability
This DyLight® 594 conjugate is stable when stored at 2-8°C. Do not freeze.
Reported Applications
FC
Each investigator should determine their own optimal working dilution for specific applications.


Country of Origin
USA
References
1. Tan, C. et al. (1987) Nucleic Acids Research 15:9299
2. Zuber, M. et al. (1989) Molec. Cell. Biol. 9:57
3. Waseem, H. N. et al. (1990) J. Cell Sci. 96:121
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