Recombinant Human GFRα-2

Glial cell line-derived neurotrophic factor family receptor alpha-2, also known as GFRA2, is the cognate co-receptor for the neurotrophic factor neurturin that is selectively expressed by isolectin B(4) (IB 4)-binding nociceptive sensory neurons.¹ GFRA2 is required for the development of the myenteric nerve plexus and is an excellent candidate gene for Hirschsprung disease (HSCR).² GFRalpha2 signaling is also needed for development of the parasympathetic islet innervation that is critical for vagally induced hormone secretion.³

Fc Fusion Protein

spsslqgpe lhgwrppvdc vranelcaae sncssryrtl rqclagrdrn tmlankecqa alevlqespl ydcrckrgmk kelqclqiyw sihlgltege efyeaspyep vtsrlsdifr lasifsgtga dpvvsaksnh cldaakacnl ndnckklrss yisicnreis ptercnrrkc hkalrqffdr vpseytyrml fcscqdqaca errrqtilps csyedkekpn cldlrgvcrt dhlcrsrlad fhancrasyq tvtscpadny qaclgsyagm igfdmtpnyv dssptgivvs pwcscrgsgn meeecekflr dftenpclrn aiqafgngtd vnvspkgpsf qatqaprvek tpslpddlsd stslgtsvit tctsvqeqgl kannskelsm cftelttnii pgsnkvikpn sdiegrmdpk scdkthtcpp cpapellggp svflfppkpk dtlmisrtpe vtcvvvdvsh edpevkfnwy vdgvevhnak tkpreeqyns tyrvvsvltv lhqdwlngke ykckvsnkal papiektisk akgqprepqv ytlppsrdel tknqvsltcl vkgfypsdia vewesngqpe nnykttppvl dsdgsfflys kltvdksrwq qgnvfscsvm healhnhytq kslslspgkh hhhhh

Ser22

Lyophilized

The predicted molecular weight of Recombinant Human GFRα-2 is Mr 74 kDa. However, the actual molecular weight as observed by migration on SDS-PAGE is Mr 100 kDa.

74

This recombinant protein was 0.2 µm filtered and lyophilized from modified Dulbecco’s phosphate buffered saline (1X PBS) pH 7.2 – 7.3 with no calcium, magnesium, or preservatives.

This lyophilized protein is stable for six to twelve months when stored desiccated at -20°C to -70°C. After aseptic reconstitution, this protein may be stored at 2°C to 8°C for one month or at -20°C to -70°C in a manual defrost freezer. Avoid Repeated Freeze Thaw Cycles. See Product Insert for exact lot specific storage instructions.

Next Day Ambient

1. Lewin, GR. et al. (2002) J Physiol. 545: 43
2. Mulligan, LM. et al. (2001) Human Genetics 108: 409
3. Herzig, KH. et al. (2005) Diabetes 54: 1324