Recombinant Human PDGF Rα

Platelet-derived growth factor receptor, alpha polypeptide, also known as PDGFRA, encodes a cell surface tyrosine kinase receptor for members of the platelet-derived growth factor family. These growth factors are mitogens for cells of mesenchymal origin. The identity of the growth factor bound to a receptor monomer determines whether the functional receptor is a homodimer or a heterodimer, composed of both platelet-derived growth factor receptor alpha and beta polypeptides. A soluble form of the platelet-derived growth factor (PDGF) alpha receptor, designated sPDGF-R alpha is capable of binding PDGF ligand and can compete with cell-associated PDGF receptors for ligand binding.¹

The biological activity of Human PDGF sRα was determined by its ability to inhibit the biological activity of hPDGF-AB or rhPDGF-AA in quiescent NR6R-3T3 fibroblasts (Raines, E. W. et al., 1985, Methods in Enzymology 109:749-773). The ED₅₀ for this effect is typically 5.0 - 10.0 μg/ml in the presence of 10 ng/ml rhPDGF-AA.

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Gln24 predicted

Lyophilized

The predicted molecular weight of Recombinant Human PDGF Rα is Mr 56 kDa. However, the actual molecular weight as observed by migration on SDS-PAGE is Mr 95-107 kDa.

56

This recombinant protein was 0.2 µm filtered and lyophilized from modified Dulbecco’s phosphate buffered saline (1X PBS) pH 7.2 – 7.3 with no calcium, magnesium, or preservatives.

This lyophilized protein is stable for six to twelve months when stored desiccated at -20°C to -70°C. After aseptic reconstitution, this protein may be stored at 2°C to 8°C for one month or at -20°C to -70°C in a manual defrost freezer. Avoid Repeated Freeze Thaw Cycles. See Product Insert for exact lot specific storage instructions.

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1. Hart, CE. et al. (1993) J Biol Chem. 268: 9621
2. Martinho, O. et al. (2009) Br. J. Cancer 101:973.