Recombinant Human CD120b (TNFR2)

Tumor necrosis factor receptor II (TNF-RII, TNFRSF1B, p75, CD120b) is a membrane glycoprotein in the TNF receptor superfamily. It is present on most cell types and is considered to play a prominent role in stimulation by TNF-α (1). TNF-RII proteins are expressed by hematopoietic cells including macrophages, neutrophils, lymphocytes, thymocytes and mast cells, as well as endothelial cells, cardiac myocytes and prostate cells (2). Soluble TNF-RII neutralizes the biological activities of TNF-α and TNF-β with equal efficiency (3).

Fc Fusion Protein

lpaqvaft pyapepgstc rlreyydqta qmccskcspg qhakvfctkt sdtvcdsced stytqlwnwv peclscgsrc ssdqvetqac treqnrictc rpgwycalsk qegcrlcapl rkcrpgfgva rpgtetsdvv ckpcapgtfs nttsstdicr phqicnvvai pgnasrdavc tstsptrsma pgavhlpqpv strsqhtqpt pepstapsts fllpmgpspp aegstgdieg rmdpkscdkt htcppcpape llggpsvflf ppkpkdtlmi srtpevtcvv vdvshedpev kfnwyvdgve vhnaktkpre eqynstyrvv svltvlhqdw lngkeykckv snkalpapie ktiskakgqp repqvytlpp srdeltknqv sltclvkgfy psdiavewes ngqpennykt tppvldsdgs fflyskltvd ksrwqqgnvf scsvmhealh nhytqkslsl spgkhhhhhh

Leu23

Lyophilized

The predicted molecular weight of Recombinant Human TNF RII is Mr 52.6 kDa. However, the actual molecular weight as observed by migration on SDS Page is Mr 70 kDa.

52.6

This recombinant protein was 0.2 µm filtered and lyophilized from modified Dulbecco’s phosphate buffered saline (1X PBS) pH 7.2 – 7.3 with no calcium, magnesium, or preservatives.

This lyophilized protein is stable for six to twelve months when stored desiccated at -20°C to -70°C. After aseptic reconstitution, this protein may be stored at 2°C to 8°C for one month or at -20°C to -70°C in a manual defrost freezer. Avoid Repeated Freeze Thaw Cycles. See Product Insert for exact lot specific storage instructions.

Next Day Ambient

1. Zuckerman, KS. et al. (1998) Cancer Res. 58:2217
2. Schall, TJ. et al. (1990) Cell 61:361
3. Tartaglia, LA. et al. (1992) Immunol. Today 13:151