Recombinant Human VEGF R2

Vascular endothelial growth factor receptor 2 (VEGF R2) is a transmembrane glycoprotein that is a member of a receptor tyrosine kinase family whose activation plays an essential role in a large number of biological processes such as embryonic development, wound healing, cell proliferation, migration, and differentiation.^1,2,3

The biological activity of Human KDR was determined by its ability to inhibit the VEGF-dependent proliferation of human umbilical vein endothelial cells. The expected ED₅₀ for this effect is typically 4-8 ng/ml in the presence of 5 ng/ml recombinant human VEGF₁₆₅.

Fc Fusion Protein

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Ala20

Lyophilized

The predicted molecular weight of Recombinant Human KDR is Mr 110 kDa. However, the actual molecular weight as observed by migration on SDS-PAGE is Mr 160-170 kDa.

110

This recombinant protein was 0.2 µm filtered and lyophilized from modified Dulbecco’s phosphate buffered saline (1X PBS) pH 7.2 – 7.3 with no calcium, magnesium, or preservatives.

This lyophilized protein is stable for six to twelve months when stored desiccated at -20°C to -70°C. After aseptic reconstitution, this protein may be stored at 2°C to 8°C for one month or at -20°C to -70°C in a manual defrost freezer. Avoid Repeated Freeze Thaw Cycles. See Product Insert for exact lot specific storage instructions.

Next Day Ambient

1. Homqvist, K. et al. (2004) J Biol Chem. 279: 22267
2. Meyer, RD. et al. (2002) J Biol Chem. 277: 27081
3. Rahimi, N. et al. (2000) J Biol Chem. 275: 16986