Recombinant Human GITR Ligand
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BackgroundActivation-induced TNFR member ligand (AITRL) is a member of the TNF superfamily. It is expressed in endothelial cells and along with its receptor, AITR, seems to regulate interactions between endothelial cells and activated T lymphocytes. AITRL is a type II transmembrane protein containing 177 amino acid residues. Protein DetailsPurity >97% by SDS-PAGE and analyzed by silver stain. Endotoxin Level <0.1 EU/µg as determined by the LAL method Biological Activity The biological activity of Human AITRL is determined by its ability to stimulate IL-8 production by human PBMC using a concentration range of 1.0-10.0 ng/ml. Results may vary with PBMC donors. Fusion Protein Tag Histidine Tag Protein Accession No. Amino Acid Sequence hhhhh hetakepcma kfgplpskwq masseppcvn kvsdwkleil qnglyliygq vapnanyndv apfevrlykn kdmiqtltnk skiqnvggty elhvgdtidl ifnsehqvlk nntywgiill anpqfis
N-terminal Sequence Analysis His State of Matter Lyophilized Predicted Molecular Mass The predicted molecular weight of Recombinant Human AITRL is Mr 15 kDa. However, the actual molecular weight as observed by migration on SDS-PAGE is Mr 14-16 kDa. Predicted Molecular Mass 15 Formulation This recombinant protein was 0.2 µm filtered and lyophilized from modified Dulbecco’s phosphate buffered saline (1X PBS) pH 7.2 – 7.3 with no calcium, magnesium, or preservatives. Storage and Stability The lyophilized protein should be stored desiccated at -20°C. The reconstituted protein can be stored for at least one week at 4°C. For long-term storage of the reconstituted protein, aliquot into working volumes and store at -20°C in a manual defrost freezer. Avoid Repeated Freeze Thaw Cycles. Country of Origin USA Shipping Next Day Ambient NCBI Gene Bank IMPORTANT Use lot specific datasheet for all technical information pertaining to this recombinant protein. |
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