Recombinant Human beta-Defensin 3

Recombinant Human beta-Defensin 3

Product No.: B626

[product_table name="All Top" skus="B626"]

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Alternate Names
DEFB-3, BD-3
Product Type
Recombinant Protein
Expression Host
E. coli Cells
Species
Human
Applications
ELISA Cap
FC

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Product Size
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Protein Details

Purity
>95% by SDS-PAGE and analyzed by silver stain.
Endotoxin Level
<0.01EU/µg as determined by the LAL method
Biological Activity
Measured by its anti-microbial activity against E. coli.
Protein Accession No.
Amino Acid Sequence
giintlqkyy crvrggrcav lsclpkeeqi gkcstrgrkc crrkk
N-terminal Sequence Analysis
Gly23
State of Matter
Lyophilized
Predicted Molecular Mass
The predicted molecular weight of Recombinant Human BD-3 is Mr 5.2 kDa.
Predicted Molecular Mass
5.2
Formulation
This recombinant protein was lyophilized from a 0.2 μm filtered solution in 35% acetonitrile (CH3CN) and 0.1% trifluoroacetic acid (TFA).
Storage and Stability
This lyophilized protein is stable for six to twelve months when stored desiccated at -20°C to -70°C. After aseptic reconstitution, this protein may be stored at 2°C to 8°C for one month or at -20°C to -70°C in a manual defrost freezer. Avoid Repeated Freeze Thaw Cycles. See Product Insert for exact lot specific storage instructions.
Country of Origin
USA
Shipping
Next Day Ambient
NCBI Gene Bank
Applications and Recommended Usage ?
(Quality Tested by Leinco)
ELISA Sandwich: This antibody is useful as the capture antibody in a sandwich ELISA. The suggested coating concentration is 5 µg/ml (100 µl/well) µg/ml.
Flow Cytometry: PN:A106
Flow Cytometry: It is recommended to use the indirect method for signal enhancement when enumerating cells expressing CXCR5. A suggested method would be to stain cells expressing CXCR5 with approximately 10 µl per test. A typical test sample constitutes approximately 50 µl of packed whole blood or 1 x 105 continuous passage or activated cell cultures that have been centrifuged at 500 X g for five minutes. Labeling of the cells with the biotin conjugate should be followed by PN:A104, resuspended in 200-400 µl of 1X PBS.
Elisa Sandwich Protocol
Flow Cytometry
IMPORTANT Use lot specific datasheet for all technical information pertaining to this recombinant protein.
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Prod No.
Description
B626
Products are for research use only. Not for use in diagnostic or therapeutic procedures.