Donkey F(ab’)₂ Anti-Mouse IgG (H&L) (Adsorbed Against: Ms., Hu., Rt., Rb., Shp., Hs., GP., CK., Sy. Hms.) – Biotin

Purified Mouse IgG (H&L) (>98%)

This secondary antibody is lyophilized from 0.01M phosphate buffered saline (PBS) pH 7.6 containing 0.25M NaCl, 15 mg/ml IgG free BSA and 0.05% sodium azide as a preservative.

This F(ab')₂ secondary antibody was generated by optimal pepsin degestion of the antisera followed by antigen affinity chromatography to elimiate the presence of Fc fragment and whole molecules.

This lyophilized secondary antibody conjugate should be stored desiccated at 2-8°C. The reconstituted Biotin conjugate containing glycerol should be stored at -20°C in a manual defrost freezer.

One year from date of reconstitution

Next Day Ambient

It is recommended that working dilutions be prepared fresh daily.

WB: 1:20,000 - 1:400,000
ELISA: 1:20,000 - 1:400,000
IHC: 1:500 - 1:5,000
FC: 1:200 - 1:1,0000

Secondary antibody concentrations reconstituted with glycerol will be diluted by 50%. Therefore, the working dilution may be one half of what is recommended.

Donkey F(ab')₂ Anti-Mouse IgG (H&L) recognizes Mouse IgG heavy and light chains. This secondary antibody was purified using antigen affinity chromatography and adsorbed against serum proteins and immunoglobulins to minimize species cross-reactivity.