Life science researcher performing ELISA test pipette onto plate

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Principle structure of an immunoassay: Sandwich ELISA

Types of ELISA Tests

Direct ELISA diagram
Indirect ELISA diagram
Sandwich ELISA diagram
Competitive ELISA diagram
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Types of ELISA

Direct ELISA

  • A labeled primary antibody binds to the target

Indirect ELISA

  • The most common protocol
  • The target is bound by the primary antibody, and detected when a labeled secondary antibody binds to the primary antibody

Sandwich ELISA

  • Used to detect sample antigen
  • The target is adsorbed to the assay plate, then bound by two primary antibodies

Competitive ELISA

  • Often used for smaller targets that may have only one binding site
  • Detection through competitive binding