Recombinant Mouse ALK-1

Recombinant Mouse ALK-1

Product No.: A349

[product_table name="All Top" skus="A349"]

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Alternate Names
Activin Receptor-Like Kinase 1, ACVRL1, ACVRLK1, HHT, HHT2, ORW2, SKR3, TSR-I
Product Type
Recombinant Protein
Expression Host
NS0 Cells
Species
Mouse
Applications
ELISA Cap

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Product Size
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Background

Activin A receptor type II-like 1, also known as ACVRL1, is a human gene. ACVRL1 is a receptor in the TGF beta signaling pathway. It is also known as "activin receptor-like kinase 1", or "ALK1". This gene encodes a type I cell-surface receptor for the TGF-beta superfamily of ligands. It shares with other type I receptors a high degree of similarity in serine-threonine kinase subdomains, a glycine- and serine-rich region (called the GS domain) preceding the kinase domain, and a short C-terminal tail. The encoded protein, sometimes termed ALK1, shares similar domain structures with other closely related ALK or activin receptor-like kinase proteins that form a subfamily of receptor serine/threonine kinases. Mutations in this gene are associated with hemorrhagic telangiectasia type 2, also known as Rendu-Osler-Weber syndrome 2 suggesting a critical role for ALK-1 in the control of blood vessel development or repair.

Human and mouse ALK-1 share approximately 71% amino acid sequence identity in their extracellular regions.

Protein Details

Purity
>95% by SDS-PAGE and analyzed by silver stain.
Endotoxin Level
<0.1 EU/µg as determined by the LAL method
Biological Activity
Measured by its ability to inhibit recombinant Human BMP-9 induced alkaline phosphatase production by ATDC5 cells (David, L., et al., 2007, Blood, 109:1953 - 61). The ED<sub>50</sub> for this effect is 20 - 80 ng/ml in the presence of 2 ng/ml of recombinant Human BMP-9.
Fusion Protein Tag
Fc Fusion Protein
Protein Accession No.
Amino Acid Sequence
dlrrpskl vnctcesphc krpfcqgswc tvvlvreqgr hpqvyrgcgs lnqelclgrp teflnhhccy rsfcnhnvsl mleatqtpse epevdahlpi egrmdpkscd kthtcppcpa pellggpsvf lfppkpkdtl misrtpevtc vvvdvshedp evkfnwyvdg vevhnaktkp reeqynstyr vvsvltvlhq dwlngkeykc kvsnkalpap iektiskakg qprepqvytl ppsrdeltkn qvsltclvkg fypsdiavew esngqpenny kttppvldsd gsfflysklt vdksrwqqgn vfscsvmhea lhnhytqksl slspgk
N-terminal Sequence Analysis
Asp23
State of Matter
Lyophilized
Predicted Molecular Mass
The predicted molecular weight of Recombinant Mouse ALK-1 is Mr 37.7 kDa. However, the actual molecular weight as observed by migration on SDS Page is Mr 50-55 kDa.
Predicted Molecular Mass
37.7
Formulation
This recombinant protein was 0.2 µm filtered and lyophilized from modified Dulbecco’s phosphate buffered saline (1X PBS) pH 7.2 – 7.3 with no calcium, magnesium, or preservatives.
Storage and Stability
This lyophilized protein is stable for six to twelve months when stored desiccated at -20°C to -70°C. After aseptic reconstitution, this protein may be stored at 2°C to 8°C for one month or at -20°C to -70°C in a manual defrost freezer. Avoid Repeated Freeze Thaw Cycles. See Product Insert for exact lot specific storage instructions.
Country of Origin
USA
Shipping
Next Day Ambient
NCBI Gene Bank
Elisa Sandwich Protocol
IMPORTANT Use lot specific datasheet for all technical information pertaining to this recombinant protein.
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Prod No.
Description
A349
Products are for research use only. Not for use in diagnostic or therapeutic procedures.