Recombinant Mouse E-Cadherin

Recombinant Mouse E-Cadherin

Product No.: C1355

[product_table name="All Top" skus="C1355"]

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Alternate Names
Cell-CAM120/80, Uvomorulin, Arc-1, L-CAM, CDH1, CD324, CDHE, UVO
Product Type
Recombinant Protein
Expression Host
NS0 Cells
Species
Mouse

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Product Size
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Background

Cadherin 1, type 1, E-cadherin (epithelial), also known as CDH1 and CD32, is a tumor suppressor gene. 1, 2 The encoded protein is a calcium dependent cell-cell adhesion glycoprotein comprised of five extracellular cadherin repeats, a transmembrane region and a highly conserved cytoplasmic tail. E-cadherin-mediated cell-cell adhesion pathway may represent a novel chemotherapeutic target for bladder cancer, prostate cancer, and renal-cell carcinoma. 3

Protein Details

Purity
>95% by SDS Page and analyzed by silver stain.
Endotoxin Level
<0.1 EU/µg as determined by the LAL method
Biological Activity
The biological activity of Mouse E-CAD was determined by the ability of the immobilized protein to support the adhesion of the MCF7 human breast adenocarcinoma cells. When 5 x 10<sup>4</sup> cells/well are added to rmECAD coated plates (1.5 μg/ml with 100 μl/well), approximately 50-70% will adhere after 90 minutes at room temperature.
Fusion Protein Tag
Fc Fusion Protein
Protein Accession No.
Amino Acid Sequence
dwvi ppiscpenek gefpknlvqi ksnrdketkv fysitgqgad kppvgvfiie retgwlkvtq pldreaiaky ilyshavssn geavedpmei vitvtdqndn rpeftqevfe gsvaegavpg tsvmkvsatd adddvntyna aiaytivsqd pelphknmft vnrdtgvisv ltsgldresy ptytlvvqaa dlqgeglstt akavitvkdi ndnapvfnps tyqgqvpene vnariatlkv tdddapntpa wkavytvvnd pdqqfvvvtd pttndgilkt akgldfeakq qyilhvrven eepfegslvp statvtvdvv dvneapifmp aerrvevped fgvgqeitsy tarepdtfmd qkityriwrd tanwleinpe tgaiftraem dredaehvkn styvaliiat ddgspiatgt gtlllvlldv ndnapipepr nmqfcqrnpq phiitildpd lppntspfta elthgasvnw tieyndaaqe slilqprkdl eigeykihlk ladnqnkdqv ttldvhvcdc egtvnncmka givaaglqvi egrmdpkscd kthtcppcpa pellggpsvf lfppkpkdtl misrtpevtc vvvdvshedp evkfnwyvdg vevhnaktkp reeqynstyr vvsvltvlhq dwlngkeykc kvsnkalpap iektiskakg qprepqvytl ppsrdeltkn qvsltclvkg fypsdiavew esngqpenny kttppvldsd gsfflysklt vdksrwqqgn vfscsvmhea lhnhytqksl slspgkhhhh hh
N-terminal Sequence Analysis
Asp157
State of Matter
Lyophilized
Predicted Molecular Mass
The predicted molecular weight of Recombinant Mouse ECAD is Mr 62 kDa. However, the actual molecular weight as observed by migration on SDS-PAGE is Mr 75-87 kDa.
Predicted Molecular Mass
62
Formulation
This recombinant protein was 0.2 µm filtered and lyophilized from a sterile solution containing 50mM Tris-Citrate, 50 mM NaCl and 2 mM CaCl2, pH 6.5.
Storage and Stability
This lyophilized protein is stable for six to twelve months when stored desiccated at -20°C to -70°C. After aseptic reconstitution, this protein may be stored at 2°C to 8°C for one month or at -20°C to -70°C in a manual defrost freezer. Avoid Repeated Freeze Thaw Cycles. See Product Insert for exact lot specific storage instructions.
Country of Origin
USA
Shipping
Next Day Ambient
NCBI Gene Bank

References & Citations

1. Christofori, G. et al. (1998) Am. J. Hum. Genet. 63: 1588
2. Gumbiner, BM. et al. (2003) J. Cell Biol.161: 1191
3. Isaacs, WB. et al. (1995) World J Urol. 13:364
IMPORTANT Use lot specific datasheet for all technical information pertaining to this recombinant protein.
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Products are for research use only. Not for use in diagnostic or therapeutic procedures.