Anti-Mouse CXCL1 (Clone 48415)
Anti-Mouse CXCL1 (Clone 48415)
Product No.: C1420
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Clone 48415 Target CXCL1 Formats AvailableView All Product Type Monoclonal Antibody Alternate Names GRO1, GROa, MGSA, MGSA Alpha, NAP-3, SCYB1 Isotype IgG2a Applications ELISA Cap , N , WB |
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Antibody DetailsProduct DetailsReactive Species Mouse Host Species Rat Immunogen Purified Recombinant Mouse CXCL1 (>98%) Endotoxin Level <0.1 EU/µg as determined by the LAL method Formulation This monoclonal antibody has been 0.2 µm filtered and lyophilized from modified Dulbecco's phosphate buffered saline (1X PBS) pH 7.2 - 7.4 containing 5.0% w/v trehalose with no calcium, magnesium or preservatives present. Storage and Handling The lyophilized antibody can be stored desiccated at -20°C to -70°C for up to twelve months. The reconstituted antibody can be stored for at least four weeks at 2-8°C. For long-term storage of the reconstituted antibody, aseptically aliquot into working volumes and store at -20°C to -70°C in a manual defrost freezer. Avoid repeated freeze thaw cycles. No detectable loss of activity was observed after six months. Country of Origin USA Shipping Next Day Ambient RRIDAB_2828480 Applications and Recommended Usage? Quality Tested by Leinco ELISA Sandwich: This antibody is useful as the capture antibody in a sandwich ELISA. The suggested coating concentration is 2-8 µg/ml. A suitable detection antibody is PN:K124 at a concentration of approximately 0.1 - 0.4 µg/ml. A suggested standard for this assay is PN:K144. Western Blotting: To detect Mouse CXCL1 this monoclonal antibody can be used at a concentration of 1-2 µg/ml. This monoclonal antibody should be used in conjunction with compatible second-step reagents such as PN:R377 and a chromogenic substrate such as PN:T343. The detection limit for Mouse CXCL1 is 25 ng/lane under either reducing or non-reducing conditions. The sensitivity of detection may increase up to 50 fold when a chemiluminescent substrate is used. Additional Applications Reported In Literature ? Neutralization: This antibody is useful for neutralization of Mouse CXCL1 bioactivity. The antibody dose required to neutralize 50% (ND50) of the biological activity of Mouse CXCL1 (at 30 ng/ml) is 0.05 - 0.25 µg/ml. Each investigator should determine their own optimal working dilution for specific applications. See directions on lot specific datasheets, as information may periodically change. DescriptionSpecificity Rat Anti-Mouse CXCL1 (Clone 48415) recognizes an epitope on Mouse CXCL1. This monoclonal antibody was purified using multi-step affinity chromatography methods such as Protein A or G depending on the species and isotype. Background Chemokine (C-X-C motif) ligand 1 (CXCL1) is a small cytokine with mitogenic properties belonging to the CXC chemokine family that was previously called GRO1 oncogene, GROα, KC, Neutrophil-activating protein 3 (NAP-3) and melanoma growth stimulating activity, alpha (MSGA-α).1 CXCL1 is expressed by macrophages, neutrophils and epithelial cells.2 CXCL1 plays a role in spinal cord development by inhibiting the migration of oligodendrocyte precursors and is involved in the processes of angiogenesis, inflammation, wound healing.3,4 Antigen DetailsPubMed References & Citations1. Sager, R. et al. (1990) Proc. Natl. Acad. Sci. U.S.A. 87: 7732 2. Grotendorst, GR. et al. (1990) Mol. Cell. Biol. 10: 5596 3. Miller, RH. et al. (2002) Cell 110: 373 4. Richmond, A. et al. (2000) J. Invest. Dermatol. 115: 234 Technical Protocols N  |
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Formats Available
Products are for research use only. Not for use in diagnostic or therapeutic procedures.
