Anti-Mouse CCL27 – Biotin
Anti-Mouse CCL27 – Biotin
Product No.: C1520
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Target CCL27 Product Type Polyclonal Antibody Alternate Names Chemokine (C-C Motif) Ligand 27 , ALP, ILC, Eskine, Cutaneous T-Cell Attracting Chemokine, CTACK, SCYA27, skinkine, PESKY Applications ELISA Det , WB |
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Antibody DetailsProduct DetailsReactive Species Mouse Host Species Goat Immunogen Purified Recombinant Mouse CCL27 (Accession # NP_035466) Formulation This biotinylated antigen affinity purified polyclonal antibody has been 0.2 µm filtered and lyophilized from modified Dulbecco’s phosphate buffered saline (1X PBS) pH 7.2 – 7.4 containing 50 µg of bovine serum albumin per µg of antibody with no calcium, magnesium, or preservatives present. State of Matter Lyophilized Storage and Handling The lyophilized, biotinylated antigen affinity purified polyclonal antibody can be stored desiccated at -20°C to -70°C for up to twelve months from date of receipt. The reconstituted biotin conjugate can be stored for at least four weeks at 2-8°C. For long-term storage of the reconstituted conjugate, aseptically aliquot into working volumes and store at -20°C to -70°C in a manual defrost freezer. Avoid Repeated Freeze Thaw Cycles. No detectable loss of activity was observed after six months. Country of Origin USA Shipping Next Day Ambient RRIDAB_2828590 Applications and Recommended Usage? Quality Tested by Leinco Western Blotting: To detect Mouse CCL27 this biotin conjugate can be used at a concentration of 0.1 - 0.2 µg/ml. This biotin conjugate should be used in conjunction with compatible second-step reagents such as PN:A106 and a chromogenic substrate such as PN:T343. The detection limit for Mouse CCL27 is 20 ng/lane under either reducing or non-reducing conditions. The sensitivity of detection may increase up to 50 fold when a chemiluminescent substrate is used. A suitable Western blotting control is PN:C359. ELISA Sandwich Assay: This antibody can be used as the detection antibody in a sandwich ELISA at a concentration of approximately 0.1-0.4 µg/mL when used in conjunction with PN:C1452 as the capture antibody at 2 µg/ml and an optimal second step reagent such as PN:A106 for the detection of Mouse CCL27. Each investigator should determine their own optimal working dilution for specific applications. See directions on lot specific datasheets, as information may periodically change. DescriptionSpecificity Goat Anti-Mouse Cutaneous T-Cell Attracting Chemokine (CTACK) recognizes Mouse CTACK. This antigen affinity purified polyclonal antibody was purified using a proprietary chromatographic technique that includes covalently immobilizing the antigen proteins or peptides to agarose based beads. This purification method enhances specificity, reduces nonspecific binding of extraneous IgG and provides you with the most reliable reagent available for your early discovery research. Background Chemokine (C-C motif) ligand 27 (CCL27) is a small cytokine belonging to the CC chemokine family. It is associated with homing of memory T lymphocytes to the skin, and plays a role in T cell-mediated inflammation of the skin. CCL27 is expressed in numerous tissues, including gonads, thymus, placenta and skin. It elicits its chemotactic effects by binding to the chemokine receptor CCR10. CCL27 is one of the CC chemokines produced by epidermal keratinocytes and is suggested to be involved in the pathogenesis of inflammatory skin diseases. CCL27 modifies inflammation by attracting CCR10-positive and CCR4-positive cells into the lesional skin, and may participate in the pathogenesis of Th2-shifted skin diseases such as atopic dermatitis. Antigen DetailsPubMed NCBI Gene Bank ID UniProt.org Technical ProtocolsELISA Det  |
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Products are for research use only. Not for use in diagnostic or therapeutic procedures.
