Recombinant Human CD23

Recombinant Human CD23

Product No.: C1591

[product_table name="All Top" skus="C1591"]

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Alternate Names
CD23 Antigen, Fc-epsilon-RII, Lymphocyte IgE receptor, BLAST-2
Product Type
Recombinant Protein
Expression Host
NS0 Cells
Species
Human
Applications
ELISA Cap

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Product Size
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Background

CD23, also known as Fc-ε-RII/BLAST-2, is a low affinity IgE Fc receptor which belongs to the C-type lectin structural family and plays a role in the regulation of IgE synthesis and IgE mediated activities (1). It is distinct from the high affinity IgE receptors found on basophils and mast cells, which mediate allergic reactions. CD23 is found both as a transmembrane receptor protein and in a soluble form, which is generated by proteolytic cleavage of membrane bound CD23 (2). It is expressed on most mature, conventional B cells (but not on peritoneal CD5+ B cells), and can also be found on the surface of T cells, macrophages, platelets, eosinophils and EBV transformed B lymphoblasts (3). Expression of CD23 has also been detected in neoplastic cells from cases of B cell chronic Lymphocytic leukemia (4). CD23 is expressed by B cells in the follicular mantle but not by proliferating germinal centre cells.

Protein Details

Purity
>90% by SDS-PAGE and analyzed by silver stain.
Endotoxin Level
<0.1 EU/µg as determined by the LAL method
Fusion Protein Tag
Histidine Tag
Protein Accession No.
Amino Acid Sequence
dtt qslkqleera arnvsqvskn leshhgdqma qksqstqisq eleelraeqq rlksqdlels wnlnglqadl ssfksqelne rneasdller lreevtklrm elqvssgfvc ntcpekwinf qrkcyyfgkg tkqwvharya cddmegqlvs ihspeeqdfl tkhashtgsw iglrnldlkg efiwvdgshv dysnwapgep tsrsqgedcv mmrgsgrwnd afcdrklgaw vcdrlatctp pasegsaesm gpdsrpdpdg rlptpsaplh shhhhhhhhh
N-terminal Sequence Analysis
His
State of Matter
Lyophilized
Predicted Molecular Mass
The predicted molecular weight of Recombinant Human CD23 is Mr 32 kDa. However, the actual molecular weight as observed by migration on SDS-PAGE is Mr 41 kDa.
Predicted Molecular Mass
32
Formulation
This recombinant protein was 0.2 µm filtered and lyophilized from modified Dulbecco’s phosphate buffered saline (1X PBS) pH 7.2 – 7.3 with no calcium, magnesium, or preservatives.
Storage and Stability
This lyophilized protein is stable for six to twelve months when stored desiccated at -20°C to -70°C. After aseptic reconstitution, this protein may be stored at 2°C to 8°C for one month or at -20°C to -70°C in a manual defrost freezer. Avoid Repeated Freeze Thaw Cycles. See Product Insert for exact lot specific storage instructions.
Country of Origin
USA
Shipping
Next Day Ambient
NCBI Gene Bank

References & Citations

1. Kijimoto-Ochiai, S. et al. (2002) Cell. Mol. Life Sci. 59:648
2. Visan, I. et al. (2003) Euro. J. Immunol. 33:1163
3. Abbas, AK. et al. (2003) Cellular and Molecular Immunology, Saunders
4. Mossalayi, MD. et al. (1991) Annls. New York Acad. Sci. 628:362
Elisa Sandwich Protocol
IMPORTANT Use lot specific datasheet for all technical information pertaining to this recombinant protein.
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Products are for research use only. Not for use in diagnostic or therapeutic procedures.