Recombinant Human FGF-17

Fibroblast growth factor 17, also known as FGF17 is a member of the fibroblast growth factor (FGF) family. FGF family members possess broad mitogenic and cell survival activities, and are involved in a variety of biological processes including embryonic development cell growth, morphogenesis, tissue repair, tumor growth and invasion. FGF-17 is most similar to FGF-8 and is highly conserved between mouse and human (93 % identity).^1,2 FGF17 is required for several complex social behaviors and studies suggest that disturbances in FGF17 signaling may contribute to neuropsychiatric diseases that affect such behaviors.³

The biological activity of Human FGF-17 was determined by its ability to stimulate 3H-thymidine incorporation by NR6R-3T3 fibroblast. The expected ED₅₀ for this effect is typically 15 - 60 ng/ml, in the presence of 1 μg/ml heparin.

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Thr23 (with and without an N-terminal Met)

Lyophilized

The predicted molecular weight of Recombinant Human FGF-17 is Mr 22.6 kDa. However, the actual molecular weight as observed by migration on SDS-PAGE is 21.5 and 22.6 kDa (reducing conditions).

22.6

This recombinant protein was 0.2 µm filtered and lyophilized from modified Dulbecco’s phosphate buffered saline (1X PBS) pH 7.2 – 7.3 with no calcium, magnesium, or preservatives.

This lyophilized protein is stable for six to twelve months when stored desiccated at -20°C to -70°C. After aseptic reconstitution, this protein may be stored at 2°C to 8°C for one month or at -20°C to -70°C in a manual defrost freezer. Avoid Repeated Freeze Thaw Cycles. See Product Insert for exact lot specific storage instructions.

Next Day Ambient

1. Hoshikawa, M. et al. (1998) Biochemical and Biophysical Research Communications 244: 187
2. Xu, J. et al. (1999) Mechanisms of Development 83: 165
3. Mucke, L. et al. (2008) Genes Brain Behav. 7: 344