Recombinant Human GITR

GITR (glucocorticoid-induced tumor necrosis factor receptor) also known as TNFRSF18, is a surface receptor molecule that has been shown to be involved in inhibiting the suppressive activity of T-regulatory cells and extending the survival of T-effector cells.¹ GITR is involved in the regulation of T-cell receptor mediated cell death. Constitutive expression of a transfected GITR gene induces resistance to apoptosis induced by anti CD3 monoclonal antibodies.

GITR ligand interferes with the suppressor activity of CD4(+) CD25(+) regulatory T-cells.²

Measured by its ability to bind GITR ligand. Immobilized Human GITR at 2 μg/ml (100 μl/well) can bind Human GITR ligand with a linear range of 0.3 - 20 ng/ml in a functional ELISA assay.

Fc Fusion Protein

qrptg gpgcgpgrll lgtgtdarcc rvhttrccrd ypgeeccsew dcmcvqpefh cgdpccttcr hhpcppgqgv qsqgkfsfgf qcidcasgtf sggheghckp wtdctqfgfl tvfpgnkthn avcvpgsppa e (Thr45Ala) iegrmdpks cdkthtcppc papellggps vflfppkpkd tlmisrtpev tcvvvdvshe dpevkfnwyv dgvevhnakt kpreeqynst yrvvsvltvl hqdwlngkey kckvsnkalp apiektiska kgqprepqvy tlppsrdelt knqvsltclv kgfypsdiav ewesngqpen nykttppvld sdgsfflysk ltvdksrwqq gnvfscsvmh ealhnhytqk slslspgk

No results obtained: Gln26 predicted

Lyophilized

The predicted molecular weight of Recombinant Human GITR is Mr 41 kDa. However, the actual molecular weight as observed by migration on SDS-PAGE is Mr 50 kDa.

41

This recombinant protein was 0.2 µm filtered and lyophilized from modified Dulbecco’s phosphate buffered saline (1X PBS) pH 7.2 – 7.3 with no calcium, magnesium, or preservatives.

This lyophilized protein is stable for ≥ 12 months when stored desiccated at -20°C to -70°C. After aseptic reconstitution, this protein may be stored at 2°C to 8°C for one month or at -20°C to -70°C in a manual defrost freezer. Avoid Repeated Freeze Thaw Cycles. See Product Insert for exact lot specific storage instructions.

Next Day Ambient

1. Shimizu, J. et al. (2002) Nature Immunology 3:135–142.
2. Ji, Hb. et al. (2004)Journal of Immunology 172: 5823