Recombinant Human CCL1

Recombinant Human CCL1

Product No.: I-224

[product_table name="All Top" skus="I-224"]

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Alternate Names
SCYA1, I-309, T Cell Activation Gene 3 (TCA3), P500, SISe
Product Type
Recombinant Protein
Expression Host
E. coli Cells
Species
Human

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Product Size
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Background

Chemokine (C-C motif) ligand 1 (CCL1) is a small glycoprotein secreted by activated T cells that belongs to a family inflammatory cytokines known as chemokines.1 CCL1 attracts monocytes, NK cells, and immature B cells and dendritic cells by interacting with a cell surface chemokine receptor called CCR8.2 CCL1 may play a role in lymphocyte recruitment in bronchial asthma.3

Protein Details

Purity
>97% by SDS-PAGE and analyzed by silver stain.
Endotoxin Level
<0.01EU/µg as determined by the LAL method
Biological Activity
The biological activity of Human I-309 was determined by by its ability to chemoattract mouse BW5147 cells or hCCR8 transfected BaF/3 cells. The expected ED<sub>50</sub> for these effects are typically 3 - 9 ng/ml and 1.5 - 7.5 ng/ml, respectively.
Protein Accession No.
Amino Acid Sequence
ksmqvpfsrc cfsfaeqeip lrailcyrnt ssicsnegli fklkrgkeac aldtvgwvqr hrkmlrhcps krk
N-terminal Sequence Analysis
Lys24
State of Matter
Lyophilized
Predicted Molecular Mass
The predicted molecular weight of Recombinant Human CCL1 is Mr 8.5 kDa.
Predicted Molecular Mass
8.5
Formulation
This recombinant protein was lyophilized from a 0.2 μm filtered solution in 35% acetonitrile (CH3CN) and 0.1% trifluoroacetic acid (TFA).
Storage and Stability
This lyophilized protein is stable for six to twelve months when stored desiccated at -20°C to -70°C. After aseptic reconstitution, this protein may be stored at 2°C to 8°C for one month or at -20°C to -70°C in a manual defrost freezer. Avoid Repeated Freeze Thaw Cycles. See Product Insert for exact lot specific storage instructions.
Country of Origin
USA
Shipping
Next Day Ambient

References & Citations

1. Krangel, MS. et al. (1992) Proceedings of the National Acad of Sci. 89: 2950
2. Roos, RS. et al. (1997) J Biol Chem. 272: 17251
3. Teran, LM. et al. (2006) Eur Respir J. 28: 59
IMPORTANT Use lot specific datasheet for all technical information pertaining to this recombinant protein.
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Products are for research use only. Not for use in diagnostic or therapeutic procedures.