Recombinant Human IGFBP-3

Insulin-like growth factor binding protein 3, also known as IGFBP3, is a member of the insulin-like growth factor binding protein (IGFBP) family that are cysteine-rich proteins that act as a carrier protein. The protein forms a ternary complex with insulin-like growth factor acid-labile subunit (IGFALS) and either insulin-like growth factor (IGF) I or II. In this form, it circulates in the plasma, prolonging the half-life of IGFs and altering their interaction with cell surface receptors.

The biological activity of Human IGFBP-3 was determined by its ability to inhibit the biological activity of rhIGF-I or rhIGF-II on MCF-7 cells (Karey, K.P. et al., 1988, Cancer Research 48:4083). The expected ED₅₀ for this effect is typically 0.05 - 0.15 μg/ml in the presence of 14 ng/ml rhIGF-II.

gas sgglgpvvrc epcdaralaq capppavcae lvrepgcgcc ltcalsegqp cgiytercgs glrcqpspde arplqalldg rglcvnasav srlrayllpa ppapgnases eedrsagsve spsvssthrv sdpkfhplhs kiiiikkgha kdsqrykvdy esqstdtqnf sseskretey gpcrremedt lnhlkflnvl sprgvhipnc dkkgfykkkq crpskgrkrg fcwcvdkygq plpgyttkgk edvhcysmqs k

Met

Lyophilized

The predicted molecular weight of Recombinant Human IGFBP-3 is Mr 29 kDa. However, the actual molecular weight as observed by migration on SDS-PAGE is Mr 37-47 kDa.

29

This recombinant protein was lyophilized from a 0.2 μm filtered solution in 30% acetonitrile (CH₃CN) and 0.1% trifluoroacetic acid (TFA).

This lyophilized protein is stable for six to twelve months when stored desiccated at -20°C to -70°C. After aseptic reconstitution, this protein may be stored at 2°C to 8°C for one month or at -20°C to -70°C in a manual defrost freezer. Avoid Repeated Freeze Thaw Cycles. See Product Insert for exact lot specific storage instructions.

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