Recombinant Human IL-28A

Interleukin-28A (IL-28A), also known as IFN-λ2, is a cytokine distantly related to type I interferons and the IL-10 family. It exerts its biological effects through a receptor that also binds IL-28B and IL-29 (1). This receptor is a heterodimeric class II cytokine receptor that consists of IL-10RB and IL-28RA. IL-28A activities overlap those of type I IFNs and include anti-viral activity and up-regulation of MHC class I antigen expression (2). The expression of IL-28A is induced by virus infection or double-stranded RNA. Upon binding to the receptor, it also induces JAK kinase activation and phosphorylation of STAT and STAT2 (3). IL-28A represents a potential novel approach for therapy of Th1-mediated inflammatory diseases such as T cell-mediated hepatitis (4).

The biological activity of Human IFN-λ2 was determined by in an anti-viral assay using human HepG2 cells infected with encephalomyocarditis virus (Sheppard, P. et al., 2003, Nature Immunol. 4:63). The expected ED₅₀ for this effect is typically 1 - 5 ng/ml.

Histidine Tag

NP_742150

vpvar lhgalpdarg chiaqfksls pqelqafkra kdaleeslll kdcrchsrlf prtwdlrqlq vrerpmalea elaltlkvle atadtdpalv dvldqplhtl hhilsqfrac iqpqptagpr trgrlhhwly rlqeapkkes pgcleasvtf nlfrlltrdl ncvasgdlcv dihhhhhh

Val26

Lyophilized

The predicted molecular weight of Recombinant Human IL-28A is Mr 20.8 kDa. However, the actual molecular weight as observed by migration on SDS-PAGE is Mr 24 kDa.

20.8

This recombinant protein was 0.2 µm filtered and lyophilized from modified Dulbecco’s phosphate buffered saline (1X PBS) pH 7.2 – 7.3 with no calcium, magnesium, or preservatives.

This lyophilized protein is stable for six to twelve months when stored desiccated at -20°C to -70°C. After aseptic reconstitution, this protein may be stored at 2°C to 8°C for one month or at -20°C to -70°C in a manual defrost freezer. Avoid Repeated Freeze Thaw Cycles. See Product Insert for exact lot specific storage instructions.

Next Day Ambient

1. Zitzmann, K. et al. (2006) Biochem. Biophys. Research Commun. 344:1334
2. Kotenko, SV. et al. (2003) Nat. Immunol. 4:69
3. Maher, SG. et al. (2008) Cancer Biol. Ther. 7:1109
4. Neurath, MF. et al. (2007) Gastroenterology 132:358