Recombinant Human IGF-I R

eicgpgidir ndyqqlkrle nctviegylh illiskaedy rsyrfpkltv iteylllfrv agleslgdlf pnltvirgwk lfynyalvif emtnlkdigl ynlrnitrga irieknadlc ylstvdwsli ldavsnnyiv gnkppkecgd lcpgtmeekp mcekttinne ynyrcwttnr cqkmcpstcg kractennec chpeclgscs apdndtacva crhyyyagvc vpacppntyr fegwrcvdrd fcanilsaes sdsegfvihd gecmqecpsg firngsqsmy cipcegpcpk vceeekktkt idsvtsaqml qgctifkgnl linirrgnni aselenfmgl ievvtgyvki rhshalvsls flknlrlilg eeqlegnysf yvldnqnlqq lwdwdhrnlt ikagkmyfaf npklcvseiy rmeevtgtkg rqskgdintr nngerasces dvlhftsttt sknriiitwh ryrppdyrdl isftvyykea pfknvteydg qdacgsnswn mvdvdlppnk dvepgillhg lkpwtqyavy vkavtltmve ndhirgakse ilyirtnasv psipldvlsa snsssqlivk wnppslpngn lsyyivrwqr qpqdgylyrh nycskdkipi rkyadgtidi eevtenpkte vcggekgpcc acpkteaekq aekeeaeyrk vfenflhnsi fvprperkrr dvmqvanttm ssrsrnttaa dtynitdpee leteypffes rvdnkertvi snlrpftlyr idihscnhea eklgcsasnf vfartmpaeg addipgpvtw eprpensifl kwpepenpng lilmyeikyg sqvedqrecv srqeyrkygg aklnrlnpgn ytariqatsl sgngswtdpv ffyvqaktgy en (single chain) eicgpgidir ndyqqlkrle nctviegylh illiskaedy rsyrfpkltv iteylllfrv agleslgdlf pnltvirgwk lfynyalvif emtnlkdigl ynlrnitrga irieknadlc ylstvdwsli ldavsnnyiv gnkppkecgd lcpgtmeekp mcekttinne ynyrcwttnr cqkmcpstcg kractennec chpeclgscs apdndtacva crhyyyagvc vpacppntyr fegwrcvdrd fcanilsaes sdsegfvihd gecmqecpsg firngsqsmy cipcegpcpk vceeekktkt idsvtsaqml qgctifkgnl linirrgnni aselenfmgl ievvtgyvki rhshalvsls flknlrlilg eeqlegnysf yvldnqnlqq lwdwdhrnlt ikagkmyfaf npklcvseiy rmeevtgtkg rqskgdintr nngerasces dvlhftsttt sknriiitwh ryrppdyrdl isftvyykea pfknvteydg qdacgsnswn mvdvdlppnk dvepgillhg lkpwtqyavy vkavtltmve ndhirgakse ilyirtnasv psipldvlsa snsssqlivk wnppslpngn lsyyivrwqr qpqdgylyrh nycskdkipi rkyadgtidi eevtenpkte vcggekgpcc acpkteaekq aekeeaeyrk vfenflhnsi fvprperkrr (α chain) dvmqvanttm ssrsrnttaa dtynitdpee leteypffes rvdnkertvi snlrpftlyr idihscnhea eklgcsasnf vfartmpaeg addipgpvtw eprpensifl kwpepenpng lilmyeikyg sqvedqrecv srqeyrkygg aklnrlnpgn ytariqatsl sgngswtdpv ffyvqaktgy en (β chain)

Glu31 and Asp741

Lyophilized

The predicted molecular weight of Recombinant Human IGF-I R is Mr 102.9 kDa (single chain), 81.0 kDa (α chain) & 21.9 kDa (β chain). However, the actual molecular weight as observed by migration on SDS-PAGE is 160 kDa, 125 kDa and 35 & 40 kDa under reducing conditions.

This recombinant protein was 0.2 µm filtered and lyophilized from modified Dulbecco’s phosphate buffered saline (1X PBS) pH 7.2 – 7.3 with no calcium, magnesium, or preservatives.

This lyophilized protein is stable for six to twelve months when stored desiccated at -20°C to -70°C. After aseptic reconstitution, this protein may be stored at 2°C to 8°C for one month or at -20°C to -70°C in a manual defrost freezer. Avoid Repeated Freeze Thaw Cycles. See Product Insert for exact lot specific storage instructions.

Next Day Ambient

ELISA Sandwich: This antibody is useful as the capture antibody in a sandwich ELISA. The suggested coating concentration is 5 µg/ml (100 µl/well) µg/ml.
Flow Cytometry: PN:A106
Flow Cytometry: It is recommended to use the indirect method for signal enhancement when enumerating cells expressing CXCR5. A suggested method would be to stain cells expressing CXCR5 with approximately 10 µl per test. A typical test sample constitutes approximately 50 µl of packed whole blood or 1 x 10⁵ continuous passage or activated cell cultures that have been centrifuged at 500 X g for five minutes. Labeling of the cells with the biotin conjugate should be followed by PN:A104, resuspended in 200-400 µl of 1X PBS.