Anti-Rat MIP-3α (Clone 83225)
Anti-Rat MIP-3α (Clone 83225)
Product No.: M1280
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Clone 83225 Target MIP-3 alpha Formats AvailableView All Product Type Monoclonal Antibody Alternate Names CKb4, Liver Activation Regulated Chemokine (LARC), MIP3A, SCYA20, ST38, Exodus-1, CCL20 Isotype IgG1 Applications ELISA Cap , N , WB |
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Antibody DetailsProduct DetailsReactive Species Rat Host Species Mouse Immunogen Purified Recombinant Rat MIP-3α (>98%) Endotoxin Level <0.1 EU/µg as determined by the LAL method Formulation This monoclonal antibody has been 0.2 µm filtered and lyophilized from modified Dulbecco's phosphate buffered saline (1X PBS) pH 7.2 - 7.3 containing 5.0% w/v trehalose with no calcium, magnesium or preservatives present. Storage and Handling The lyophilized antibody can be stored desiccated at -20°C to -70°C for up to twelve months. The reconstituted antibody can be stored for at least four weeks at 2-8°C. For long-term storage of the reconstituted antibody, aseptically aliquot into working volumes and store at -20°C to -70°C in a manual defrost freezer. Avoid repeated freeze thaw cycles. No detectable loss of activity was observed after six months. Country of Origin USA Shipping Next Day Ambient RRIDAB_2831275 Applications and Recommended Usage? Quality Tested by Leinco ELISA Sandwich: This antibody is useful as the capture antibody in a sandwich ELISA. The suggested coating concentration is 2-8 µg/ml. A suitable detection antibody is PN:M1311 at a concentration of approximately 100 ng/ml. A suggested standard for this assay is PN:M1244. Western Blotting: To detect Rat MIP-3α this monoclonal antibody can be used at a concentration of 1-2 µg/ml. This monoclonal antibody should be used in conjunction with compatible second-step reagents such as PN:M114 and a chromogenic substrate such as PN:T343. The detection limit for Rat MIP-3α is 25 ng/lane under either reducing or non-reducing conditions. The sensitivity of detection may increase up to 50 fold when a chemiluminescent substrate is used. Additional Applications Reported In Literature ? Neutralization: This antibody is useful for neutralization of Rat MIP-3α bioactivity. The antibody dose required to neutralize 50% (ND50) of the biological activity of Rat MIP-3α (at 5 ng/ml) is 20 - 40 µg/ml. Each investigator should determine their own optimal working dilution for specific applications. See directions on lot specific datasheets, as information may periodically change. DescriptionSpecificity Mouse Anti-Rat Macrophage Inflammatory Protein 3 Alpha (MIP-3α) (Clone 83225) recognizes an epitope on Rat MIP-3α. This monoclonal antibody was purified using multi-step affinity chromatography methods such as Protein A or G depending on the species and isotype. Background Macrophage Inflammatory Protein-3 Alpha (MIP3A) also known as CCL20 or liver activation regulated chemokine (LARC) is a small cytokine belonging to the CC chemokine family. It is strongly chemotactic for lymphocytes and weakly attracts neutrophils.1 MIP3A is specifically expressed by the follicle-associated epithelia (FAE) covering intestinal Peyer's patches (PPs) and is the only known chemokine ligand for the chemokine receptor CCR6.2 MIP3-alpha is produced by intestinal epithelial cells (including cells associated with mucosal lymphoid tissues), by keratinocytes in inflamed skin, and by cells in liver and lung.3,4 It plays an important role in the inflammatory response of blood monocytes and tissue macrophages. Antigen DetailsPubMed References & Citations1. Nomiyama, H. et al. (1997) J. Biol. Chem. 272: 5846 2. Kucharzik, T. et al. (2005) J. Pathol. 166: 1647 3. Cook, DN. et al. (2000) Immunity 12: 495 4. Farber, JM. et al. (2002) J. Immunol. 168: 4871 Technical Protocols N  |
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Formats Available
Products are for research use only. Not for use in diagnostic or therapeutic procedures.
