Recombinant Human MIP-4 (CCL18)

Macrophage Inflammatory Protein-4 (MIP-4) produced in E.Coli is a single, non-glycosylated polypeptide that attracts lymphocytes but not monocytes or granulocytes and is involved in B cell migration into B cell follicles in lymph nodes. It attracts naive T lymphocytes toward dendritic cells and activated macrophages in lymph nodes, has chemotactic activity for naive T cells, CD4+ and CD8+ T cells and thus may play a role in both humoral and cell-mediated immunity responses. MIP-4 is expressed at high levels in lung, lymph nodes, placenta, and bone marrow, not expressed by peripheral blood monocytes, and a monocyte-to-macrophage differentiation is a prerequisite for expression.

The biological activity of Human MIP-4 was determined by by its ability to induce chemotaxis of resting T-lymphocytes and its ability to inhibit rhEotaxin induced chemotaxis on rat Y3 cells transfected with human CCR3 (Nibbs, RJB. et al., 2000, J. Immunol. 164:1488). The expected ED₅₀ for these effects is typically 0.5 - 2 μg/ml and 1 - 5 μg/ml, respectively.

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Ala21

Lyophilized

The predicted molecular weight of Recombinant Human MIP-4 is Mr 7.8 kDa.

7.8

This recombinant protein was lyophilized from a 0.2 μm filtered solution in 30% acetonitrile (CH₃CN) and 0.1% trifluoroacetic acid (TFA).

This lyophilized protein is stable for six to twelve months when stored desiccated at -20°C to -70°C. After aseptic reconstitution, this protein may be stored at 2°C to 8°C for one month or at -20°C to -70°C in a manual defrost freezer. Avoid Repeated Freeze Thaw Cycles. See Product Insert for exact lot specific storage instructions.

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1. Nomiyama, H. et al. (1997) J. Biol. Chem. 272: 5846
2. Kucharzik, T. et al. (2005) J. Pathol. 166: 1647