Anti-Human TGF-α
Anti-Human TGF-α
Product No.: T145
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Target TGF-alpha Product Type Polyclonal Antibody Alternate Names Transforming Growth Factor-Alpha, Sacroma Growth Factor, TGF-Type I, ETGF, MDGF-2 (Milk-Derived Growth Factor-2), TCGF (Transformed Cell Growth Factor) Applications ELISA Cap , IHC FFPE , N , WB |
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Antibody DetailsProduct DetailsReactive Species Human Host Species Goat Immunogen Purified Recombinant Human TGF-α (>98%) Endotoxin Level <0.1 EU/µg as determined by the LAL method Formulation This antigen affinity purified polyclonal antibody has been 0.2 µm filtered and lyophilized from modified Dulbecco’s phosphate buffered saline (1X PBS) pH 7.2 – 7.4 containing 5.0% w/v trehalose with no calcium, magnesium, or preservatives present. State of Matter Lyophilized Storage and Handling The lyophilized antigen affinity purified polyclonal antibody can be stored desiccated at -20°C to -70°C for twelve months from date of receipt. The reconstituted antibody can be stored for at least four weeks at 2-8°C. For long-term storage of the reconstituted antibody, aseptically aliquot into working volumes and store at -20°C to -70°C in a manual defrost freezer. Avoid Repeated Freeze Thaw Cycles. No detectable loss of activity was observed after six months. Country of Origin USA Shipping Next Day Ambient RRIDAB_2831827 Applications and Recommended Usage? Quality Tested by Leinco ELISA Sandwich: This antibody is useful as the capture antibody in a sandwich ELISA. The suggested coating concentration is 0.2-0.8 µg/ml. A suitable detection antibody is PN:T186 at a concentration of approximately 0.1-0.4 µg/ml. A suggested standard for this assay is PN:T151. Western Blotting: To detect Human TGF-α this polyclonal antibody can be used at a concentration of 0.1-0.2 µg/ml. This polyclonal antibody should be used in conjunction with compatible second-step reagents such as PN:G505 and a chromogenic substrate such as PN:T343. The detection limit for Human TGF-α is 50 and 25 ng/lane under either reducing or non-reducing conditions. The sensitivity of detection may increase up to 50 fold when a chemiluminescent substrate is used. A suitable Western blotting control is PN:T151. Additional Applications Reported In Literature ? IHC (NBF/Par.): This antibody should give satisfactory staining results when used at a concentration of 15 µg/ml. The recommended secondary antibody for IHC is PN:G505. For chromogenic detection with high signal and low background use PN:D100 or PN:K107. Neutralization: Measured by its ability to neutralize TGF‑ alpha -induced proliferation in the Balb/3T3 mouse embryonic fibroblast cell line. The Neutralization Dose) is typically 0.15-0.75 μg/mL in the presence of 3 ng/mL Recombinant Human TGF‑ alpha. Each investigator should determine their own optimal working dilution for specific applications. See directions on lot specific datasheets, as information may periodically change. DescriptionSpecificity Rabbit Anti-Human Transforming Growth Factor-Alpha (TGF-α) recognizes Human TGF-α. This antigen affinity purified polyclonal antibody was purified using a proprietary chromatographic technique that includes covalently immobilizing the antigen proteins or peptides to agarose based beads. This purification method enhances specificity, reduces nonspecific binding of extraneous IgG and provides you with the most reliable reagent available for your early discovery research. Background Transforming growth factor alpha (TGF-α) is thought to be the major autocrine factor controlling growth in epidermal cells.1 It plays an important role in cell proliferation during embryogenesis.2 It also stimulates neural cell proliferation in the adult injured brain.3 TGF-alpha is an oncogenic growth factor which drives estrogen independent breast tumor proliferation via an autocrine loop.4 TGF-alpha which normally suppresses tumours can actually be used by cancer cells to enhance their ability to spread. It is present in high levels in around half of all breast tumours, and these tumours tend to be more aggressive and more likely to spread to the lungs. It has been shown that TGF-alpha can prompt breast cancer cells to make another cytokine called angiopoietin-like 4 (ANGPTL4), which helps the cells to spread through the bloodstream.5 Antigen DetailsPubMed NCBI Gene Bank ID UniProt.org References & Citations1. Taylor, HS. et al. (2009) Semin Reprod Med.27(1):62-79 2. Lamaroon, A. et al. (2002) J Dental Research 75:1534 3. Loughlin, S. et al. (2002) Proc Natl Acad Sci USA 97:14686 4. Fuchs, E. et al. (1991) Genes & Dev. 5:714 Technical Protocols IHC FFPE N  |
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Products are for research use only. Not for use in diagnostic or therapeutic procedures.
