Anti-Mouse CCL1 (Clone 148107)

Anti-Mouse CCL1 (Clone 148107)

Product No.: T459

[product_table name="All Top" skus="T459"]

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Clone
148107
Target
CCL1
Formats AvailableView All
Product Type
Monoclonal Antibody
Alternate Names
Chemokine (C-C Motif) Ligand 1, SCYA1, I-309, T Cell Activation Gene 3 (TCA3), P500, SISe
Isotype
IgG2b
Applications
ELISA Cap
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N

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Product Size
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Antibody Details

Product Details

Reactive Species
Mouse
Host Species
Rat
Immunogen
Purified Recombinant Mouse CCL1 (>98%)
Endotoxin Level
<0.1 EU/µg as determined by the LAL method
Formulation
This monoclonal antibody has been 0.2 µm filtered and lyophilized from modified Dulbecco's phosphate buffered saline (1X PBS) pH 7.2 - 7.3 containing 5.0% w/v trehalose with no calcium, magnesium or preservatives present.
Storage and Handling
The lyophilized antibody can be stored desiccated at -20°C to -70°C for up to twelve months. The reconstituted antibody can be stored for at least four weeks at 2-8°C. For long-term storage of the reconstituted antibody, aseptically aliquot into working volumes and store at -20°C to -70°C in a manual defrost freezer. Avoid repeated freeze thaw cycles. No detectable loss of activity was observed after six months.
Country of Origin
USA
Shipping
Next Day Ambient
Applications and Recommended Usage?
Quality Tested by Leinco
ELISA Sandwich: This antibody is useful as the capture antibody in a sandwich ELISA. The suggested coating concentration is 2-8 µg/ml. A suitable detection antibody is PN:T504 at a concentration of approximately 0.1-0.4 µg/ml. A suggested standard for this assay is PN:T410.
Additional Applications Reported In Literature ?
Neutralization: This antibody is useful for neutralization of Mouse TCA-3 bioactivity. The antibody dose required to neutralize 50% (ND50) of the biological activity of Mouse TCA-3 (at 10 ng/ml) is 0.6 - 3.0 µg/ml.
Each investigator should determine their own optimal working dilution for specific applications. See directions on lot specific datasheets, as information may periodically change.

Description

Specificity
Rat Anti-Mouse Chemokine (C-C Motif) Ligand 1 (CCL1) (Clone 148107) recognizes an epitope on Mouse CCL1. This monoclonal antibody was purified using multi-step affinity chromatography methods such as Protein A or G depending on the species and isotype.
Background
Chemokine (C-C motif) ligand 1 (CCL1) is a small glycoprotein secreted by activated T cells that belongs to a family inflammatory cytokines known as chemokines.1 CCL1 attracts monocytes, NK cells, and immature B cells and dendritic cells by interacting with a cell surface chemokine receptor called CCR8.2 CCL1 may play a role in lymphocyte recruitment in bronchial asthma.3

Antigen Details

PubMed
NCBI Gene Bank ID

References & Citations

1. Krangel, MS. et al. (1992) Proceedings of the National Acad of Sci. 89: 2950
2. Roos, RS. et al. (1997) J Biol Chem. 272: 17251
3. Teran, LM et al. (2006) Eur Respir J. 28: 59
Elisa Sandwich Protocol
N
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Formats Available

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Prod No.
Description
T459
Products are for research use only. Not for use in diagnostic or therapeutic procedures.