Anti-Rat TIMP-1 – Biotin

Anti-Rat TIMP-1 – Biotin

Product No.: T494

[product_table name="All Top" skus="T494"]

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Target
TIMP-1
Product Type
Polyclonal Antibody
Alternate Names
Tissue Inhibitor of Metalloproteinase 1, EPO, CLGI, EPA, FLJ90373, HCI, TIMP
Applications
ELISA Det
,
WB

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Antibody Details

Product Details

Reactive Species
Rat
Host Species
Goat
Immunogen
Purified Recombinant Rat TIMP-1 (Accession # P30120)
Formulation
This biotinylated antigen affinity purified polyclonal antibody has been 0.2 µm filtered and lyophilized from modified Dulbecco’s phosphate buffered saline (1X PBS) pH 7.2 – 7.3 containing 50 µg of bovine serum albumin per µg of antibody with no calcium, magnesium, or preservatives present.
State of Matter
Lyophilized
Storage and Handling
The lyophilized, biotinylated antigen affinity purified polyclonal antibody can be stored desiccated at -20°C to -70°C for up to twelve months from date of receipt. The reconstituted biotin conjugate can be stored for at least four weeks at 2-8°C. For long-term storage of the reconstituted conjugate, aseptically aliquot into working volumes and store at -20°C to -70°C in a manual defrost freezer. Avoid Repeated Freeze Thaw Cycles. No detectable loss of activity was observed after six months.
Country of Origin
USA
Shipping
Next Day Ambient
Applications and Recommended Usage?
Quality Tested by Leinco
Western Blotting: To detect Rat TIMP-1 this biotin conjugate can be used at a concentration of 0.1 - 0.2 µg/ml. This biotin conjugate should be used in conjunction with compatible second-step reagents such as PN:A106 and a chromogenic substrate such as PN:T343. The detection limit for Rat TIMP-1 is 2 ng/lane under either reducing or non-reducing conditions. The sensitivity of detection may increase up to 50 fold when a chemiluminescent substrate is used. A suitable Western blotting control is PN:T399.
ELISA Sandwich Assay: This antibody can be used as the detection antibody in a sandwich ELISA at a concentration of approximately 0.1-0.4 µg/mL when used in conjunction with PN:T446 as the capture antibody at 2 µg/ml and an optimal second step reagent such as PN:A106 for the detection of Rat TIMP-1.
Each investigator should determine their own optimal working dilution for specific applications. See directions on lot specific datasheets, as information may periodically change.

Description

Specificity
Goat Anti-Rat Tissue Inhibitor of Metalloproteinases 1 (TIMP-1) recognizes Rat TIMP-1. This antigen affinity purified polyclonal antibody was purified using a proprietary chromatographic technique that includes covalently immobilizing the antigen proteins or peptides to agarose based beads. This purification method enhances specificity, reduces nonspecific binding of extraneous IgG and provides you with the most reliable reagent available for your early discovery research.
Background
Tissue Inhibitor of Metalloproteinases 1, also known as TIMP1 is a widely expressed, secreted protein that functions primarily to inhibit members of a large family of metalloproteinases (MPs). Because of the ability of TIMP-1 to inhibit MPs, it functions in many of the same pathophysiological processes as these enzymes, e.g. wound healing, ovulation, angiogenesis, and cancer cell metastasis. TIMP-1 can also stimulate proliferation and cellular anabolic processes.1 TIMP-1 is a novel negative regulator of HGF activity during liver regeneration.2 Overexpression of TIMP-1 could promote renal interstitial fibrosis through the inflammatory pathway, which might be partly induced by upregulating ICAM-1.3

Antigen Details

PubMed
NCBI Gene Bank ID

References & Citations

1. Denhardt, DT. et al. (2005) Breat Cancer Res Treat. 94: 185
2. Khokha, R. et al. (2005) Hepatology 41: 857
3. Chen, X. et al. (2008) Nephrology Dialysis Transportation 23: 1861
ELISA Det
General Western Blot Protocol
Products are for research use only. Not for use in diagnostic or therapeutic procedures.