Leinco Technologies manufactures and inventories CODEX® barcoded antibodies and complementary fluorophore-conjugated oligonucleotides (reporter molecules). Providing “Ready to Stain’ DNA barcoded antibodies minimizes user optimization for high multiplex imaging. CODEX® can detect over 40 biomarkers in an automated cyclical image acquisition process. Using the fully integrated, high multiplexing CODEX® technology allows an accurate picture of cell neighborhoods and interactions in the tissue microenvironment.
‘Ready to Stain’ DNA barcoded antibodies are validated according to tissue preparation, such as fresh frozen (FF) or fresh frozen paraffin-embedded (FFPE), and by tissue screened, such as liver, spleen, tonsil, brain, etc. Leinco DNA barcoded antibodies for CODEX® have undergone varying levels of validation and quality testing to assure conjugations are free of unconjugated antibodies and DNA barcodes.
Fluorophore reporter molecules are conjugated to complementary oligonucleotides and matched with barcoded antibodies. The four distinct fluorescent reporter molecules include Alexa Fluor™ 488 (green channel), Alexa Fluor™ 750 (infrared channel), Atto 550 (yellow channel), and Cy5 (bright red channel).