Three main components of CODEX®: Multiplex imaging makes it possible to visualize various structures in a meaningful context in order to better our understanding of biological processes. Further, CODEX® makes use of DNA barcode technology comprised of unique oligonucleotide sequences conjugated to an antibody.
Single-step staining to preserve sample integrity: CODEX® uses antibodies conjugated to unique barcodes. This enables customizable panels up to 50 antibodies, combined for a single tissue staining reaction.
An automated process of imaging biomarkers using the CODEX® instrument and a companion microscope: The CODEX® fluidics instrument has an automated imaging cycle. The first step is the staining of tissue with to up to 50 uniquely barcoded antibodies. Then, applied to the tissue and imaged are three reporter dyes with complimentary barcodes. Finally, the three reporter dyes are removed and the process is repeated until all antibodies have been imaged.