Custom Antibody Barcoding Services and CODEX® Validated Antibodies
Leinco offers custom antibody barcoding services, providing researchers with CODEX® validated antibodies conjugated to unique oligonucleotide barcodes. Undoubtedly, Leinco is bridging the gap to simplify the development and manufacturing process for CODEX® users. Leinco’s selection of In vivo GOLD™ and PLATNIUM™ functional grade antibodies optimized and validated for use with the CODEX® imaging system for spatially-resolved, highly multiplexed biomarker analysis.
Image Data: Fresh Frozen (FF) human liver tissue stained with Leinco antibodies custom barcoded for use on CODEX®, a highly multiplexed fluorescent imaging platform. (Anti-Human CD16; Clone 3G8; Leinco Prod. No. C2858) (Anti-Human CD19; Clone SJ25-C1; Leinco Prod. No. C2854)
Benefits of CODEX® (CO-Detecting by indEXing) system
- A comprehensive end-to-end solution containing the fluidics platform, assay reagents, and bioinformatics
- Benchtop footprint that integrates with existing fluorescence microscope
- The system is scalable and capable of imaging up to 50 biomarkers per run
- Preserves the sample for Region Of Interest (ROI) analysis and H&E staining
- FF and FFPE tissue compatible
The Technology
Three main components of CODEX®: Multiplex imaging makes it possible to visualize various structures in a meaningful context in order to better our understanding of biological processes. Further, CODEX® makes use of DNA barcode technology comprised of unique oligonucleotide sequences conjugated to an antibody.
Single-step staining to preserve sample integrity: CODEX® uses antibodies conjugated to unique barcodes. This enables customizable panels up to 50 antibodies, combined for a single tissue staining reaction.
An automated process of imaging biomarkers using the CODEX® instrument and a companion microscope: The CODEX® fluidics instrument has an automated imaging cycle. The first step is the staining of tissue with to up to 50 uniquely barcoded antibodies. Then, applied to the tissue and imaged are three reporter dyes with complimentary barcodes. Finally, the three reporter dyes are removed and the process is repeated until all antibodies have been imaged.
CODEX technology was pioneered in Dr. Nolan’s lab at Stanford and now patented by Akoya Biosciences: Goltsev et al., 2018, Cell 174, 968–981