Anti-Hsp70 Antibody (11116)
Anti-Hsp70 Antibody (11116)
Product No.: 11116
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Clone 2A4 Target Hsp70 Formats AvailableView All Product Type Monoclonal Isotype Mouse IgM Applications IHC , IP , WB , AM , ICC/IF |
Data
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Antibody DetailsProduct DetailsReactivity Species Human, Mouse, Rat, Fish, Avian, Amphibian, Drosophila, Yeast Host Species Mouse Immunogen Recombinant human Hsp70 expressed in E. coli. Product Concentration Lot Specific Formulation PBS, pH 7.4 and 50% glycerol. State of Matter Liquid Product Preparation PEG Purified Storage and Handling This antibody is stable for at least one (1) year at -20°C. Regulatory Status For in vitro investigational use only. Not for
use in therapeutic or diagnostic procedures. Country of Origin USA Shipping Next Day 2-8°C Applications and Recommended Usage? Quality Tested by Leinco Immunoblotting: use at 0.2ug/mL (ECL). A band of ~70 kDa is detected.
Immunofluorescence: use at 10ug/mLImmunohistochemistry/Immunocyto-chemistry: use at 10ug/mLUser should determine optimal concentrations for their applications. Positive control: Heat-shocked HeLa cell lysate. Each investigator should determine their own optimal working dilution for specific applications. See directions on lot specific datasheets, as information may periodically change. DescriptionSpecificity This antibody recognizes human,
mouse, fish, rat, avian, amphibian,
Drosophila, and yeast Hsp70, Hsc70,
and, following heat shock, Hsp72. Background Hsp70 binds ATP with high affinity and possesses a weak ATPase activity which can be stimulated by binding to unfolded proteins and synthetic peptides. Hsp70 recognizes and binds to nascent polypeptide chains as well as partially folded intermediates of proteins preventing their aggregation and misfolding. The binding of ATP triggers a critical conformational change leading to the release of the bound protein. The ability of Hsp70 to undergo cycles of binding and release from hydrophobic stretches of partially unfolded proteins is the basis for their role in a variety of intracellular functions such as protein synthesis, protein folding and oligomerization and protein transport. Antigen DetailsNCBI Gene Bank ID UniProt.org Research Area Heat Shock & Stress Proteins References & CitationsTechnical Protocols |
Formats Available
Products are for research use only. Not for use in diagnostic or therapeutic procedures.