Anti-Hsp70 Antibody (11116)

Anti-Hsp70 Antibody (11116)

Product No.: 11116

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Clone
2A4
Target
Hsp70
Formats AvailableView All
Product Type
Monoclonal
Isotype
Mouse IgM
Applications
IHC
,
IP
,
WB
,
AM
,
ICC/IF

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Data

Western Blot analysis of Rat Cell lysates showing detection of Hsp70 protein using Mouse Anti-Hsp70 Monoclonal Antibody, Clone 2A4 (11116). Load: 15 µg. Block: 1.5% BSA for 30 minutes at RT. Primary Antibody: Mouse Anti-Hsp70 Monoclonal Antibody (11116) at 1:5000 for 2 hours at RT. Secondary Antibody: Sheep Anti-Mouse IgG: HRP for 1 hour at RT.Western Blot analysis of Rat Cell lysates showing detection of Hsp70 protein using Mouse Anti-Hsp70 Monoclonal Antibody, Clone 2A4 (11116). Load: 15 µg. Block: 1.5% BSA for 30 minutes at RT. Primary Antibody: Mouse Anti-Hsp70 Monoclonal Antibody (11116) at 1:5000 for 2 hours at RT. Secondary Antibody: Sheep Anti-Mouse IgG: HRP for 1 hour at RT.
Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-HSP70 Monoclonal Antibody, Clone 2A4 (11116). Tissue: Cervical cancer cell line (HeLa). Species: Human. Fixation: 4% Formaldehyde for 15 min at RT. Primary Antibody: Mouse Anti-HSP70 Monoclonal Antibody (11116) at 1:100 for 60 min at RT. Secondary Antibody: Goat Anti-Mouse ATTO 488 at 1:100 for 60 min at RT. Counterstain: DAPI (blue) nuclear stain at 1:5000 for 5 min RT. Localization: Nucleus, Cytoplasm. Magnification: 40X.Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-HSP70 Monoclonal Antibody, Clone 2A4 (11116). Tissue: Cervical cancer cell line (HeLa). Species: Human. Fixation: 4% Formaldehyde for 15 min at RT. Primary Antibody: Mouse Anti-HSP70 Monoclonal Antibody (11116) at 1:100 for 60 min at RT. Secondary Antibody: Goat Anti-Mouse ATTO 488 at 1:100 for 60 min at RT. Counterstain: DAPI (blue) nuclear stain at 1:5000 for 5 min RT. Localization: Nucleus, Cytoplasm. Magnification: 40X.
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Antibody Details

Product Details

Reactivity Species
Human, Mouse, Rat, Fish, Avian, Amphibian, Drosophila, Yeast
Host Species
Mouse
Immunogen
Recombinant human Hsp70 expressed in E. coli.
Product Concentration
Lot Specific
Formulation
PBS, pH 7.4 and 50% glycerol.
State of Matter
Liquid
Product Preparation
PEG Purified
Storage and Handling
This antibody is stable for at least one (1) year at -20°C.
Regulatory Status
For in vitro investigational use only. Not for use in therapeutic or diagnostic procedures.
Country of Origin
USA
Shipping
Next Day 2-8°C
Applications and Recommended Usage?
Quality Tested by Leinco
Immunoblotting: use at 0.2ug/mL (ECL). A band of ~70 kDa is detected.

Immunofluorescence: use at 10ug/mLImmunohistochemistry/Immunocyto-chemistry: use at 10ug/mLUser should determine optimal concentrations for their applications.

Positive control: Heat-shocked HeLa cell lysate.
Each investigator should determine their own optimal working dilution for specific applications. See directions on lot specific datasheets, as information may periodically change.

Description

Specificity
This antibody recognizes human, mouse, fish, rat, avian, amphibian, Drosophila, and yeast Hsp70, Hsc70, and, following heat shock, Hsp72.
Background
Hsp70 binds ATP with high affinity and possesses a weak ATPase activity which can be stimulated by binding to unfolded proteins and synthetic peptides. Hsp70 recognizes and binds to nascent polypeptide chains as well as partially folded intermediates of proteins preventing their aggregation and misfolding. The binding of ATP triggers a critical conformational change leading to the release of the bound protein. The ability of Hsp70 to undergo cycles of binding and release from hydrophobic stretches of partially unfolded proteins is the basis for their role in a variety of intracellular functions such as protein synthesis, protein folding and oligomerization and protein transport.

Antigen Details

NCBI Gene Bank ID
UniProt.org
Research Area
Heat Shock & Stress Proteins

References & Citations

IHC
Immunoprecipitation Protocol
General Western Blot Protocol
AM
ICC/IF

Formats Available

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Prod No.
Description
11116
Disclaimer AlertProducts are for research use only. Not for use in diagnostic or therapeutic procedures.