Anti-Hsp90 Antibody (11107)
Data
Immunocytochemistry/Immunofluorescence analysis using Rabbit Anti-Hsp90 Polyclonal Antibody (11107). Tissue: Heat Shocked Cervical cancer cell line (HeLa). Species: Human. Fixation: 2% Formaldehyde for 20 min at RT. Primary Antibody: Rabbit Anti-Hsp90 Polyclonal Antibody (11107) at 1:100 for 12 hours at 4°C. Secondary Antibody: FITC Goat Anti-Rabbit (green) at 1:200 for 2 hours at RT. Counterstain: DAPI (blue) nuclear stain at 1:40000 for 2 hours at RT. Localization: Cytoplasm. Magnification: 100x. (A) DAPI (blue) nuclear stain. (B) Anti-Hsp90 Antibody. (C) Composite. Heat Shocked at 42°C for 1h.
Western blot analysis of Human Cell line lysates showing detection of HSP90 protein using Rabbit Anti-HSP90 Polyclonal Antibody (11107). Load: 2 µgHsp90beta. Block: 1.5% BSA for 30 minutes at RT. Primary Antibody: Rabbit Anti-HSP90 Polyclonal Antibody (11107) at 1:10000 for 2 hours at RT. Secondary Antibody: Donkey Anti-Rabbit IgG: HRP for 1 hour at RT.
Immunohistochemistry analysis using Rabbit Anti-HSP90 Polyclonal Antibody (11107). Tissue: backskin. Species: Mouse. Fixation: Bouin’s Fixative Solution. Primary Antibody: Rabbit Anti-HSP90 Polyclonal Antibody (11107) at 1:100 for 1 hour at RT. Secondary Antibody: FITC Goat Anti-Rabbit (green) at 1:50 for 1 hour at RT. Localization: Cytoplasm.
Immunocytochemistry/Immunofluorescence analysis using Rabbit Anti-Hsp90 Polyclonal Antibody (11107). Tissue: Heat Shocked Cervical cancer cell line (HeLa). Species: Human. Fixation: 2% Formaldehyde for 20 min at RT. Primary Antibody: Rabbit Anti-Hsp90 Polyclonal Antibody (11107) at 1:100 for 12 hours at 4°C. Secondary Antibody: APC Goat Anti-Rabbit (red) at 1:200 for 2 hours at RT. Counterstain: DAPI (blue) nuclear stain at 1:40000 for 2 hours at RT. Localization: Cytoplasm. Magnification: 20x. (A) DAPI (blue) nuclear stain. (B) Anti-Hsp90 Antibody. (C) Composite. Heat Shocked at 42°C for 1h. - -
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Antibody DetailsProduct DetailsReactive Species Eukaryote Host Species Rabbit Immunogen Full-length Hsp90 protein. Product Concentration Lot Specific Formulation Whole antiserum State of Matter Liquid Product Preparation Whole antiserum Storage and Handling This antibody is stable for at least one (1) year at -20°C. Regulatory Status For in vitro investigational use only. Not
for use in therapeutic or diagnostic
procedures. Country of Origin USA Shipping Next Day 2-8°C Applications and Recommended Usage? Quality Tested by Leinco Immunoblotting: use at 1:20,000- 1:40,000 (ECL). A band of ~90 kDa is detected. Each investigator should determine their own optimal working dilution for specific applications. See directions on lot specific datasheets, as information may periodically change. DescriptionDescriptionSpecificity T
This antibody recognizes eukaryotic
Hsp90αβ.
Background Hsp90 is a highly conserved and essential stress protein that is expressed in all eukaryotic cells. Hsp90 is involved in the folding, assembly, maturation, and stabilization of specific proteins as a member of a chaperone complex. It is also highly expressed in unstressed cells where it participates in controlling the activity, turnover, and trafficking of a variety of proteins. Most of the Hsp90-regulated proteins are involved in cell signaling and include the kinases, v-Src, Wee1, c-Raf, transcriptional regulators such as p53 and steroid receptors, and the polymerases of hepatitis B virus and telomerase. Function Molecular chaperone that promotes the maturation, structural maintenance and proper regulation of specific target proteins involved for instance in cell cycle control and signal transduction. Undergoes a functional cycle linked to its ATPase activity. This cycle probably induces conformational changes in the client proteins, thereby causing their activation. Interacts dynamically with various co-chaperones that modulate its substrate rPubMed:16478993, PubMed:18239673, PubMed:19696785, PubMed:20353823, PubMed:24613385, PubMed:32272059, PubMed:25973397, PubMed:26991466, PubMed:27295069}. NCBI Gene Bank ID UniProt.org Research Area Heat Shock & Stress Proteins References & CitationsTechnical Protocols    ICC/IF Certificate of Analysis |
Products are for research use only. Not for use in diagnostic or therapeutic procedures.
