Anti-Human CCL17

Anti-Human CCL17

Product No.: T166

[product_table name="All Top" skus="T166"]

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Target
CCL17
Product Type
Polyclonal Antibody
Alternate Names
Chemokine (C-C Motif) Ligand 17, Thymus and Activation Regulated Chemokine (TARC), A-152E5.3, ABCD-2, MGC138271, MGC138273, SCYA17, TARC, Dendrokine
Applications
ELISA Det
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IHC FFPE
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WB

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Product Size
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Antibody Details

Product Details

Reactive Species
Human
Host Species
Goat
Immunogen
Purified Recombinant Human TARC (>98%)
Endotoxin Level
<0.1 EU/µg as determined by the LAL method
Formulation
This antigen affinity purified polyclonal antibody has been 0.2 µm filtered and lyophilized from modified Dulbecco’s phosphate buffered saline (1X PBS) pH 7.2 – 7.4 containing 5.0% w/v trehalose with no calcium, magnesium, or preservatives present.
State of Matter
Lyophilized
Storage and Handling
The lyophilized antigen affinity purified polyclonal antibody can be stored desiccated at -20°C to -70°C for twelve months from date of receipt. The reconstituted antibody can be stored for at least four weeks at 2-8°C. For long-term storage of the reconstituted antibody, aseptically aliquot into working volumes and store at -20°C to -70°C in a manual defrost freezer. Avoid Repeated Freeze Thaw Cycles. No detectable loss of activity was observed after six months.
Country of Origin
USA
Shipping
Next Day Ambient
Applications and Recommended Usage?
Quality Tested by Leinco
Western Blotting: To detect Human CCL17 this polyclonal antibody can be used at a concentration of 0.1-0.2 µg/ml. This polyclonal antibody should be used in conjunction with compatible second-step reagents such as PN:G505 and a chromogenic substrate such as PN:T343. The detection limit for Human CCL17 is 5.0 ng/lane under either reducing or non-reducing conditions. The sensitivity of detection may increase up to 50 fold when a chemiluminescent substrate is used. A suitable Western blotting control is PN:T158.
Additional Applications Reported In Literature ?
IHC (NBF/Par.): This antibody should give satisfactory staining results when used at a concentration of 5-15 µg/ml. The recommended secondary antibody for IHC is PN:G505. For chromogenic detection with high signal and low background use PN:D100 or PN:K107.
Neutralization: This antibody is useful for neutralization of Human CCL17 bioactivity. The antibody dose required to neutralize 50% (ND50) of the biological activity of Human CCL17 (at 0.01 ng/ml) is 0.3-1.5 µg/ml.
ELISA Det
Each investigator should determine their own optimal working dilution for specific applications. See directions on lot specific datasheets, as information may periodically change.

Description

Specificity
Goat Anti-Human Chemokine (C-C Motif) Ligand 17 (CCL17) recognizes Human CCL17. This antigen affinity purified polyclonal antibody was purified using a proprietary chromatographic technique that includes covalently immobilizing the antigen proteins or peptides to agarose based beads. This purification method enhances specificity, reduces nonspecific binding of extraneous IgG and provides you with the most reliable reagent available for your early discovery research.
Background
Chemokine (C-C motif) ligand 17 (CCL17) is a small cytokine belonging to the CC chemokine and is expressed constitutively in thymus, but only transiently in phytohemagglutinin-stimulated peripheral blood mononuclear cells.1 This chemokine specifically binds and induces chemotaxis in T cells and elicits its effects by interacting with the chemokine receptor CCR4.1,2 CCL17 may be a useful serological marker and may facilitate an assessment of the degree of disease activity in systemic lupus erythematosus (SLE).3 It may also participate in the pathogenesis of skin diseases such as atopic dermatitis (AD) by regulating both allergic and irritant inflammation.4

Antigen Details

PubMed
NCBI Gene Bank ID

References & Citations

1. Yoshie, O. et al. (1996) J Biol Chem. 271: 21514
2. Yoshie, O. et al. (1997) J Biol Chem. 272: 15036
3. Kamatani, N. et al. (2003) J Rheumatology 30: 2369
4. Kunihiko, K. et al. (2006) Eur J Immunol. 36: 2116
ELISA Det
IHC FFPE
N
General Western Blot Protocol
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Products are for research use only. Not for use in diagnostic or therapeutic procedures.