PMA-induced U-937 cells. U-937 is an human lymphoma cell line that expresses markers and properties of monocytes.
This conjugate is formulated in phosphate buffered saline (PBS), 1 mg/ml BSA and 10 mM sodium azide as a preservative.
Storage and Handling
Store at 2-8°C protected from light. DO NOT FREEZE. For long term storage aliquot conjugate into working volumes and store at 2-8°C for up to a year.
Country of Origin
Next Day Ambient
Blue Laser (488 nm)
Applications and Recommended Usage?
Quality Tested by Leinco
FC The suggested concentration for this 8E11 antibody for staining cells in flow cytometry is ≤ 1 μg per 106 cells in a volume of 100 μl or 100μl of whole blood. Titration of the reagent is recommended for optimal performance for each application.
Additional Reported Applications For Relevant Conjugates ?
For specific conjugates of this clone, review literature for suggested application details.
Each investigator should determine their own optimal working dilution for specific applications. See directions on lot specific datasheets, as information may periodically change.
Mouse Anti-Human CD105 (Clone 8E11) recognizes Human CD105. This monoclonal antibody was purified using multi-step affinity chromatography methods such as Protein A or G depending on the species and isotype. Endoglin is an endothelial homodimeric membrane antigen containing the tripeptide sequence RGD, which is a recognition motif for adhesion receptors of the integrin family. It has been proposed that endoglin is a TGF-beta receptor. Endoglin is a glycoprotein present on endothelial cells of capillaries, arterioles and venules in a variety of tissues and at low levels on acute lymphoblastic and myelocytic leukemia cells.
NCBI Gene Bank ID
References & Citations
1. Lastres, P. et. al. (1994) Biochem. J. 301:765 2. Westenend, P. J. et al. (1994) J. Am. Soc. Neph. 5:822 3. Fernandez-Ruiz, E. et al. (1993) Cell Genet. 64:204 4. Bellon, T. et al. (1993) Eur. J. Immunol. 23:2340 5. Lastres, P. et al. (1992) Eur. J. Immunol. 22:393 6. O'Connell, P. J. et al. (1992) Clin. Exp. Immunol. 90:154 7. Lopez-Casillas, F. et al. (1991) Cell 67:785
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