Anti-Human CD45 (Clone F10-89-4) – FITC
Anti-Human CD45 (Clone F10-89-4) – FITC
Product No.: C198
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Clone F10-89-4 Target CD45 Formats AvailableView All Product Type Monoclonal Antibody Alternate Names LCA [Leukocyte Common Antigen] Isotype IgG2a Applications FC |
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Antibody DetailsProduct DetailsReactive Species Human Host Species Mouse Immunogen Purified T Cells from Human Lymph Nodes Formulation This conjugate is formulated in phosphate buffered saline (PBS), 1 mg/ml BSA and 10 mM sodium azide as a preservative. Storage and Handling Store at 2-8°C protected from light. DO NOT FREEZE. For long term storage aliquot conjugate into working volumes and store at 2-8°C for up to a year. Country of Origin USA Shipping Next Day Ambient Excitation Laser Blue Laser (488 nm) RRIDAB_2829065 Each investigator should determine their own optimal working dilution for specific applications. See directions on lot specific datasheets, as information may periodically change. DescriptionSpecificity Clone F10-89-4 recognizes human CD45. Antigen Distribution CD45 is expressed on all hematopoietic cells other than mature erythrocytes and platelets. Background CD45 is a 180-240kD glycoprotein member of the protein tyrosine phosphatase (PTP) family known for its involvement in regulating a variety of cellular processes including cell growth, differentiation, mitotic cycle, and oncogenic transformation. CD45 and its isoforms are vital regulators of T- and B-cell antigen receptor signaling. CD45 functions through its extracellular domain or through its cytoplasmic domain, and serves as a negative regulator of cytokine receptor signaling via JAK kinase supression. The large extracellular domain is highly glycosylated, and its multiple isoforms allow extensive variation in the structure of its side chains. CD45 isoforms show cell-type and differentiation-stage specific expression that can be used as markers that identify and distinguish between different types of immune cells. CD45R is an isoform of CD45 with a molecular weight of 220 kD. CD45R contains all three possible exons (A, B, and C); making it the longest protein generated from alternative splicing with a migration at 200 kD when isolated from T cells. Furthermore, B cells express CD45R with heavier glycosylation, bringing the molecular weight to 220 kD, hence the name B220. Notably, B220 expression is not only restricted to B cells and may also be expressed on activated T cells, on a subset of dendritic cells, and on other antigen-presenting cells. Additionally, activated and memory T lymphocytes express CD45RO which facilitates T cell activation. CD45RO lacks all three possible exons (A, B, and C), making it the shortest CD45 isoform. Antigen DetailsLigand/Receptor Galectin-1, CD2, CD3, and CD4. PubMed NCBI Gene Bank ID UniProt.org References & Citations1. Justement, L. B. et al. (1994) Immunol. Today 15:399
2. Leukocyte Typing IV (1989) Oxford University Press
3. Leukocyte Typing III (1987) Oxford University Press
4. Dalchau, R. et al. (1960) Eur. J. Immunol. 10:737 Technical Protocols |
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Formats Available
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Prod No. | Description |
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C198 | |
C197 |
Products are for research use only. Not for use in diagnostic or therapeutic procedures.