Anti-Human CD45 (Clone F10-89-4) – FITC

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Pricing & Details

Product No.C198
Clone
F10-89-4
Protein
CD45
Formats AvailableView All
Product Type
Monoclonal Antibody
Alternate Names
LCA [Leukocyte Common Antigen]
Isotype
Mouse
IgG2a
Applications
FC
Prod No.
Size
Price
Avail.
Qty
Add to cart
C198-100 tests
100 tests
$289.00
In stock
Max:
Min: 1
Step: 1
Bulk quantities available. Contact us for pricing.

Antibody Details

Product Details

Reactivity Species
Human
Host Species
Mouse
Immunogen
Purified T Cells from Human Lymph Nodes
Formulation
This conjugate is formulated in phosphate buffered saline (PBS), 1 mg/ml BSA and 10 mM sodium azide as a preservative.
Storage and Handling
Store at 2-8°C protected from light. DO NOT FREEZE. For long term storage aliquot conjugate into working volumes and store at 2-8°C for up to a year.
Country of Origin
USA
Shipping
Next Day Ambient
Excitation Laser
Blue Laser (488 nm)
Each investigator should determine their own optimal working dilution for specific applications. See directions on lot specific datasheets, as information may periodically change.

Description

Specificity
Clone F10-89-4 recognizes human CD45.
Antigen Distribution
CD45 is expressed on all hematopoietic cells other than mature erythrocytes and platelets.
Background
CD45 is a 180-240kD glycoprotein member of the protein tyrosine phosphatase (PTP) family known for its involvement in regulating a variety of cellular processes including cell growth, differentiation, mitotic cycle, and oncogenic transformation. CD45 and its isoforms are vital regulators of T- and B-cell antigen receptor signaling. CD45 functions through its extracellular domain or through its cytoplasmic domain, and serves as a negative regulator of cytokine receptor signaling via JAK kinase supression. The large extracellular domain is highly glycosylated, and its multiple isoforms allow extensive variation in the structure of its side chains. CD45 isoforms show cell-type and differentiation-stage specific expression that can be used as markers that identify and distinguish between different types of immune cells. CD45R is an isoform of CD45 with a molecular weight of 220 kD. CD45R contains all three possible exons (A, B, and C); making it the longest protein generated from alternative splicing with a migration at 200 kD when isolated from T cells. Furthermore, B cells express CD45R with heavier glycosylation, bringing the molecular weight to 220 kD, hence the name B220. Notably, B220 expression is not only restricted to B cells and may also be expressed on activated T cells, on a subset of dendritic cells, and on other antigen-presenting cells. Additionally, activated and memory T lymphocytes express CD45RO which facilitates T cell activation. CD45RO lacks all three possible exons (A, B, and C), making it the shortest CD45 isoform.

Antigen Details

Protein
Ligand/Receptor
Galectin-1, CD2, CD3, and CD4.
PubMed
NCBI Gene Bank ID

References & Citations

1. Justement, L. B. et al. (1994) Immunol. Today 15:399 2. Leukocyte Typing IV (1989) Oxford University Press 3. Leukocyte Typing III (1987) Oxford University Press 4. Dalchau, R. et al. (1960) Eur. J. Immunol. 10:737
Flow Cytometry

Formats Available

Products are for research use only. Not for use in diagnostic or therapeutic procedures.