Anti-Human CD49f (Integrin α6) (Clone 4F10) – PE

Cell Line SW/222 (Human Colorectal Carcinoma)

This conjugate is formulated in phosphate buffered saline (PBS), 1 mg/ml BSA and 10 mM sodium azide as a preservative.

Store at 2-8°C protected from light. DO NOT FREEZE. For long term storage aliquot conjugate into working volumes and store at 2-8°C for up to a year.

Next Day 2-8°C

Blue Laser (488 nm) and/or Green Laser (532 nm)/Yellow-Green Laser (561 nm)

Mouse Anti-Human CD49f (Clone 4F10) recognizes Human CD49f. This monoclonal antibody was purified using multi-step affinity chromatography methods such as Protein A or G depending on the species and isotype. This antibody is specific to the integrin alpha6 chain which is expressed on epithelial and endothelial basement membrane. The VLA alpha6 chain, which consists of a single disulphide linked 120 kDa subunit and a small doublet subunit of 25/30 kDa, is non-covalently associated with the VLA beta-chain (CD29) in VLA-6 or alpha6/beta1 complex or with integrin beta4 chain (alpha6/beta4) The antigen is also expressed on platelets, monocytes, thymocytes and T-lymphocytes.

With integrin β1 (CD29) forms VLA-6, with integrin β4 (CD104) forms a6β4 integrin; laminin and kalinin are ligands for these receptors

1. Ramaswamy, A. et al. (1995) J. Path. 176(3):227 2. Dobson, H. et al. (1994) GUT 35:1343 3. Pignatelli, M. et al. (1992) Human Pathol. 23:1159