Anti-Human CD55 (Clone 143-30) – PE

PHA Activated Peripheral Blood Mononuclear Cells

This conjugate is formulated in phosphate buffered saline (PBS), 1 mg/ml BSA and 10 mM sodium azide as a preservative.

Store at 2-8°C protected from light. DO NOT FREEZE. For long term storage aliquot conjugate into working volumes and store at 2-8°C for up to a year

Next Day 2-8°C

Blue Laser (488 nm) and/or Green Laser (532 nm)/Yellow-Green Laser (561 nm)

Mouse Anti-Human CD55 (Clone 143-30) recognizes Human CD55. This monoclonal antibody was purified using multi-step affinity chromatography methods such as Protein A or G depending on the species and isotype. Anti-Human CD55 is the complement regulatory protein decay accelerating factor (DAF). It is a 70 kDa glycoprotein (in erythrocytes) anchored in the membrane by a glycosylphosphatidylinositol tail. In other cells the apparent molecular weight is somewhat larger. It has a substantial content of O-glycans, and also one N-glycan. DAF binds to activated C4b or C3b complement fragments on the cell surface, preventing the assembly and accelerating of decay of both classical and alternative pathways. DAF has a wide distribution on cells in non-hematopoietic tissues, particularly epithelium and endothelium and is specifically found at the foetal-maternal interface in placenta. Soluble forms of DAF are found, for example, in plasma, saliva, urine, etc. FUSION PARTNER: NS1 myeloma cell line.

C3b, C4b, CD97, Coxsackie viruses

1. Holanda, K. et al. (2002) Blood 100:341 2. Leukocyte Typing V (1995) Oxford Univ. Press 3. Lubin, D. M. et al. (1989) Ann. Rev. Immunol. 7:35 4. Daniels, G. L. (1989) Vox Sang. 56:205 5. Leukocyte Typing IV (1989) Oxford Univ. Press