Anti-Human CD62E (E-Selectin) – PE

TNF-Activated Human Umbilical Vein Endothelial Cells

This conjugate is formulated in phosphate buffered saline (PBS), 1 mg/ml BSA and 10 mM sodium azide as a preservative.

Store at 2-8°C protected from light. DO NOT FREEZE. For long term storage aliquot conjugate into working volumes and store at 2-8°C for up to a year.

Next Day 2-8°C

Blue Laser (488 nm) and/or Green Laser (532 nm)/Yellow-Green Laser (561 nm)

Mouse Anti-Human CD62E (Clone 1.2B6) recognizes Human CD62E. This monoclonal antibody was purified using multi-step affinity chromatography methods such as Protein A or G depending on the species and isotype. The antibody is specific to the human antigen E-selectin (ELAM-1, 115 kDa) that is expressed on activated endothelial cells. The ligand is expressed on neutrophils and may function as an early part in neutrophil binding to the endothelium.

CD62E, also known as E-Selectin, is a type I membrane glycoprotein that belongs to the selectin family of surface molecules which is activated by cytokines and mediates the binding of leukocytes. Like other selectins, it plays an important part in inflammation. During inflammation, E-selectin plays an important part in recruiting leukocytes to the site of injury.

Siayl Lewis X (CD15s) and PSGL-1 (CD162) bind to CD62E. Recently, CLA (cutaneous lymphocyte antigen) has also been reported in interact with CD62E

1. Leukocyte Typing Workshop VI (1996) Oxford University Press 2. Leukocyte Typing Workshop V (1995) Oxford University Press 3. Bevilacqua, M. P. et al. (1993) J. Clin. Invest. 91:379 4. Groves, R. W. et al. (1991) Br. J. Dermatol. 124:117 5. Wellicome, S. M. et al. (1990) J. Immunol. 144:2558