Antibody DetailsProduct DetailsReactivity Species Human Host Species Mouse Immunogen Human platelet glycoprotein Product Concentration 0.1 mg/ml Formulation This purified monoclonal antibody is formulated in phosphate buffered saline (PBS), 1 mg/ml BSA and 10 mM sodium azide as a preservative. Storage and Handling For use within 1 month of purchase store at 2-8°C. For long term storage freeze working aliquots at -20°C in a manual defrost freezer. Avoid Repeated Freeze Thaw Cycles. Country of Origin USA Shipping Next Day Ambient RRIDAB_2829712 Applications and Recommended Usage? Quality Tested by Leinco FC The suggested concentration for this MM2/57 antibody for staining cells in flow cytometry is ≤ 1 μg per 106 cells in a volume of 100 μl or 100μl of whole blood. Titration of the reagent is recommended for optimal performance for each application. Other Applications Reported In Literature ? IHC (Frozen) IP WB Each investigator should determine their own optimal working dilution for specific applications. See directions on lot specific datasheets, as information may periodically change. DescriptionSpecificity Mouse Anti-Human CD9 (Clone MM2/57) recognizes Human CD9. This monoclonal antibody was purified using multi-step affinity chromatography methods such as Protein A or G depending on the species and isotype. This antibody reacts with a 25 kDa molecular weight band on gels of non-reduced platelet membranes and immunoprecipitates a similar band from Iodine 125 labeled platelets. This CD9 antigen belongs to the TM4 superfamily of cell surface proteins with the N- and C-terminals localized in the cytoplasm. This antibody reacts immunohistochemically on frozen sections of platelets, monocytes and pre-B cells with differential staining on lymphoid and epithelial tissues. Antigen Distribution The antigen is expressed on eosinophils, granulocytes, monocytes and numerous stromal cells. Antigen DetailsProtein Ligand/Receptor Associates with CD63, CD81, CD82 and CD36, binds PSG17 PubMed NCBI Gene Bank ID UniProt.org References & Citations1. Leukocyte Typing V (1995) Oxford University Press
2. Boucheix, C. et al. (1990) J. Biol. Chem. 266:117
3. Lanza, F. et al. (1990) J. Biol. Chem. 266:10638
4. Leukocyte Typing IV (1989) Oxford University Press Technical Protocols |
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