Human platelet glycoprotein
This purified monoclonal antibody is formulated in phosphate buffered saline (PBS), 1 mg/ml BSA and 10 mM sodium azide as a preservative.
Storage and Handling
For use within 1 month of purchase store at 2-8°C. For long term storage freeze working aliquots at -20°C in a manual defrost freezer. Avoid Repeated Freeze Thaw Cycles.
Country of Origin
Next Day Ambient
Applications and Recommended Usage?
Quality Tested by Leinco
FC The suggested concentration for this MM2/57 antibody for staining cells in flow cytometry is ≤ 1 μg per 106 cells in a volume of 100 μl or 100μl of whole blood. Titration of the reagent is recommended for optimal performance for each application.
Other Applications Reported In Literature ?
Each investigator should determine their own optimal working dilution for specific applications. See directions on lot specific datasheets, as information may periodically change.
Mouse Anti-Human CD9 (Clone MM2/57) recognizes Human CD9. This monoclonal antibody was purified using multi-step affinity chromatography methods such as Protein A or G depending on the species and isotype. This antibody reacts with a 25 kDa molecular weight band on gels of non-reduced platelet membranes and immunoprecipitates a similar band from Iodine 125 labeled platelets. This CD9 antigen belongs to the TM4 superfamily of cell surface proteins with the N- and C-terminals localized in the cytoplasm. This antibody reacts immunohistochemically on frozen sections of platelets, monocytes and pre-B cells with differential staining on lymphoid and epithelial tissues.
The antigen is expressed on eosinophils, granulocytes, monocytes and numerous stromal cells.
Associates with CD63, CD81, CD82 and CD36, binds PSG17
NCBI Gene Bank ID
References & Citations
1. Leukocyte Typing V (1995) Oxford University Press 2. Boucheix, C. et al. (1990) J. Biol. Chem. 266:117 3. Lanza, F. et al. (1990) J. Biol. Chem. 266:10638 4. Leukocyte Typing IV (1989) Oxford University Press
Products are for research use only. Not for use in diagnostic or therapeutic procedures.