Anti-Human G-CSF R – Biotin
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Antibody DetailsProduct DetailsReactive Species Human Host Species Goat Immunogen Purified Recombinant Human G-CSF R (>98%) Formulation This biotinylated antigen affinity purified polyclonal antibody has been 0.2 µm filtered and lyophilized from modified Dulbecco’s phosphate buffered saline (1X PBS) pH 7.2 – 7.3 containing 50 µg of bovine serum albumin per µg of antibody with no calcium, magnesium, or preservatives present. State of Matter Lyophilized Storage and Handling The lyophilized, biotinylated antigen affinity purified polyclonal antibody can be stored desiccated at -20°C to -70°C for up to twelve months from date of receipt. The reconstituted bioin conjugate can be stored for at least four weeks at 2-8°C. For long-term storage of the reconstituted conjugate, aseptically aliquot into working volumes and store at -20°C to -70°C in a manual defrost freezer. Avoid Repeated Freeze Thaw Cycles. No detectable loss of activity was observed after six months. Country of Origin USA Shipping Next Day Ambient RRIDAB_2830277 Applications and Recommended Usage? Quality Tested by Leinco Western Blotting: To detect Human G-CSF R this biotin conjugate can be used at a concentration of 0.1 - 0.2 µg/ml. This biotin conjugate should be used in conjunction with compatible second-step reagents such as PN:A106 and a chromogenic substrate such as PN:T343. The detection limit for Human G-CSF R is 20 ng/lane under either reducing or non-reducing conditions. The sensitivity of detection may increase up to 50 fold when a chemiluminescent substrate is used. ELISA Sandwich Assay: This antibody can be used as the detection antibody in a sandwich ELISA at a concentration of approximately 0.1-0.4 µg/ml. Each investigator should determine their own optimal working dilution for specific applications. See directions on lot specific datasheets, as information may periodically change. DescriptionSpecificity Goat Anti-Human Granulocyte Colony Stimulating Factor Soluble Receptor (G-CSF R) recognizes Human G-CSF R. This antigen affinity purified polyclonal antibody was purified using a proprietary chromatographic technique that includes covalently immobilizing the antigen proteins or peptides to agarose based beads. This purification method enhances specificity, reduces nonspecific binding of extraneous IgG and provides you with the most reliable reagent available for your early discovery research. Background Granulocyte colony-stimulating factor receptor (G-CSF R or GCSFR) is a member of the well-characterized hematopoietin receptor superfamily1 involved in the control of neutrophil development.2 GCSFR is present on precursor cells in bone marrow and in response to stimulation by G-CSF, initiates proliferation and differentiation into mature granulocytes. A number of mutations or rare polymorphisms in the GCSF-R gene have been reported in various types of myeloid diseases, such as severe congenital neutropenia (SCN)3,4 and myelodysplasia (MDS)5 Antigen DetailsNCBI Gene Bank ID UniProt.org Research Area Other Molecules References & Citations1. Bazan, JF. et al. (1990) Proc Natl Acad Sci USA 87: 6934 2. Cong, Y. et al. (2006) Cytokine 33: 219 3. Touw, IP. et al. (2002) N Engl J Med. 333: 487 4. Touw, IP. et al. (1997) Leukemia 11: 120 5. Kizaki, M. et al. (2002) J Cell Physiol. 191: 327 Technical ProtocolsELISA Det  |
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