Anti-Human Mitochondria is supplied in filtered tissue supernatant (RPMI-1640) pH-7.2 containig 0.09% sodium azide.
Storage and Handling
For long term storage freeze working aliquots at -20°C in a manual defrost freezer. Avoid Repeated Freeze Thaw Cycles.
Country of Origin
Next Day 2-8°C
Each investigator should determine their own optimal working dilution for specific applications. See directions on lot specific datasheets, as information may periodically change.
Mouse Anti-Human Mitochondria (Clone AE-1) recognizes a (Mr 60 kDa) non-glycosylated protein component of human mitochondria. The sequence of the epitope is unmapped.
In cell biology, a mitochondrion (plural mitochondria) is a membrane-enclosed organelle found in most eukaryotic cells. These organelles range from 1–10 micrometers (μm) in size. Mitochondria are sometimes described as "cellular power plants" because they generate most of the cell's supply of adenosine triphosphate (ATP), used as a source of chemical energy. In addition to supplying cellular energy, mitochondria are involved in a range of other processes, such as signaling, cellular differentiation, cell death, as well as the control of the cell cycle and cell growth. Mitochondria have been implicated in several human diseases and may play a role in the aging process. Several characteristics make mitochondria unique. The number of mitochondria in a cell varies widely by organism and tissue type. Many cells have only a single mitochondrion, whereas others can contain several thousand mitochondria. The organelle is composed of compartments that carry out specialized functions. These compartments or regions include the outer membrane, the intermembrane space, the inner membrane, and the cristae and matrix. In humans, mitochondria contain about 615 distinct types of proteins, depending on the tissue of origin. Although most of a cell's DNA is contained in the cell nucleus, the mitochondrion has its own independent genome. Further, its DNA shows substantial similarity to bacterial genomes.
References & Citations
1. Oizumi, K. et al. (1997) Br J Haematol. 99(2):415-7. Article Link
2. Ferrara, P. et al. (1996) J Biol Chem. 271(12):6941-6. Article Link
3. Chandler, James P. et al. (2011) Surg Neurol Int. 2:116. PubMed
4. Hashizume, Y. et al. (2000) Acta Neuropathol.99(1):31-8. PubMed
5. Casellas, P. et al. (1999) J Biol Chem. 274(5):2938-52. Article Link
Products are for research use only. Not for use in diagnostic or therapeutic procedures.