Anti-Mouse CD154 – Purified in vivo PLATINUM™ Functional Grade

Pricing & Details

Product No.C2357
Clone
MR1
Protein
CD154
Formats AvailableView All
Product Type
Monoclonal Antibody
Alternate Names
CD40L, TRAP, T-BAM, CD40
Isotype
Armenian Hamster
IgG
Applications
B
,
FC
,
IHC FF
,
WB
Prod No.
Size
Price
Avail.
Qty
Add to cart
C2357-0.5mg
0.5 mg
$98.00
In stock
Max:
Min: 1
Step: 1
C2357-1.0 mg
1.0 mg
$150.00
In stock
Max:
Min: 1
Step: 1
C2357-5.0 mg
5.0 mg
$495.00
In stock
Max:
Min: 1
Step: 1
C2357-50mg
50 mg
$2,200.00
In stock
Max:
Min: 1
Step: 1
Bulk quantities available. Contact us for pricing.

Antibody Details

Product Details

Reactivity Species
Mouse
Host Species
Armenian Hamster
Immunogen
Activated mouse Th1 clone D1.6
Product Concentration
1.0 - 5.0 mg/ml
Endotoxin Level
<0.5 EU/mg as determined by the LAL method
Purity
≥98% monomer by analytical SEC
>95% by SDS Page
Formulation
This monoclonal antibody is aseptically packaged and formulated in 0.01 M phosphate buffered saline (150 mM NaCl) PBS pH 7.2 - 7.4 with no carrier protein, potassium, calcium or preservatives added.
Product Preparation
Functional grade preclinical antibodies are manufactured in an animal free facility using only In vitro protein free cell culture techniques and are purified by a multi-step process including the use of protein A or G to assure extremely low levels of endotoxins, leachable protein A or aggregates.
Pathogen Testing
To protect mouse colonies from infection by pathogens and to assure that experimental preclinical data is not affected by such pathogens, all of Leinco’s Purified Functional PLATINUM™ antibodies are tested and guaranteed to be negative for all pathogens in the IDEXX IMPACT I Mouse Profile.
Storage and Handling
Functional grade preclinical antibodies may be stored sterile as received at 2-8°C for up to one month. For longer term storage, aseptically aliquot in working volumes without diluting and store at -80°C. Avoid Repeated Freeze Thaw Cycles.
Country of Origin
USA
Shipping
Next Day 2-8°C
Applications and Recommended Usage?
Quality Tested by Leinco
FC The suggested concentration for this MR1 antibody for staining cells in flow cytometry is ≤ 0.5 μg per 106 cells in a volume of 100 μl. Titration of the reagent is recommended for optimal performance for each application.
WB The suggested concentration for this MR1 antibody for use in western blotting is 1-10 μg/ml.
Other Applications Reported In Literature ?
IHC (Frozen)
B
NOTE: For most successful immunofluorescent staining results, it may be important to maximize signal over background by using a relatively bright fluorochrome-antibody conjugate or by using a high sensitivity, three-layer staining technique (e.g., including a biotinylated antibody (Part No.: 2349) or biotinylated anti-Armenian hamster IgG (Part No.: I-445) second step, followed by SA-PE (Part No.: S211).
Each investigator should determine their own optimal working dilution for specific applications. See directions on lot specific datasheets, as information may periodically change.

Description

Specificity
Clone MR1 recognizes an epitope on mouse CD154.
Antigen Distribution
CD154 is expressed on activated CD4+ T cells.
Background
CD40 is a 48 kD type I transmembrane glycoprotein that is a member of the TNFR superfamily. CD40, in association with its ligand CD154 (CD40L) - a 39 kD protein, acts as a costimulatory molecule for the activation of B cells, dendritic cells, monocytes, and other antigen presenting cells. CD40 is involved in Ig isotype switching and dendritic cell maturation, as well as the activation, differentiation and proliferation of B cells. CD40 interacts with TNFR2 and is involved in the regulation of signal transduction. CD40 is a potential target for cancer immunotherapy. Blocking the interaction of CD40 with its ligand (CD154) is the sought-after therapeutic objective for preventing and/or improving both autoimmune diseases and transplant rejection. Studies have shown that monoclonal antibodies that block CD154 in human clinical trials resulted in unanticipated vascular complications. Hence, an interest in the therapeutic potential for antagonist mAbs specific for human CD40 is emerging. Antibodies of particular therapeutic interest are those that do not inhibit CD40 signaling via physical competition with CD154. Additionally, the interaction of CD40 and its ligand (CD154) is found to be essential for amyloid-beta-induced microglial activation, thus plays a significant part in Alzheimer disease pathogenesis.

Antigen Details

Protein
Ligand/Receptor
CD40
PubMed
NCBI Gene Bank ID
Flow Cytometry
IHC
General Western Blot Protocol

Formats Available

Products are for research use only. Not for use in diagnostic or therapeutic procedures.