Anti-Mouse CD1d (Clone D5) – Purified in vivo GOLD™ Functional Grade

Pricing & Details

Product No.C2451
Clone
D5
Protein
CD1d
Formats AvailableView All
Product Type
Monoclonal Antibody
Alternate Names
R3G1
Isotype
Mouse
IgG2b κ
Applications
IP
,
WB
Prod No.
Size
Price
Avail.
Qty
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C2451-1 mg
1 mg
$105.00
In stock
Max:
Min: 1
Step: 1
C2451-5 mg
5 mg
$355.00
In stock
Max:
Min: 1
Step: 1
C2451-25 mg
25 mg
$965.00
In stock
Max:
Min: 1
Step: 1
C2451-50 mg
50 mg
$1,595.00
In stock
Max:
Min: 1
Step: 1
C2451-100 mg
100 mg
$2,300.00
In stock
Max:
Min: 1
Step: 1
Bulk quantities available. Contact us for pricing.

Antibody Details

Product Details

Reactivity Species
Mouse
Host Species
Mouse
Immunogen
CD1d a1-a2 domains
Product Concentration
≥ 5.0 mg/ml
Endotoxin Level
< 1.0 EU/mg as determined by the LAL method
Purity
≥95% monomer by analytical SEC
>95% by SDS Page
Formulation
This monoclonal antibody is aseptically packaged and formulated in 0.01 M phosphate buffered saline (150 mM NaCl) PBS pH 7.2 - 7.4 with no carrier protein, potassium, calcium or preservatives added. Due to inherent biochemical properties of antibodies, certain products may be prone to precipitation over time. Precipitation may be removed by aseptic centrifugation and/or filtration.
Product Preparation
Functional grade preclinical antibodies are manufactured in an animal free facility using only In vitro protein free cell culture techniques and are purified by a multi-step process including the use of protein A or G to assure extremely low levels of endotoxins, leachable protein A or aggregates.
Storage and Handling
Functional grade preclinical antibodies may be stored sterile as received at 2-8°C for up to one month. For longer term storage, aseptically aliquot in working volumes without diluting and store at -80°C. Avoid Repeated Freeze Thaw Cycles.
Country of Origin
USA
Shipping
Next Day 2-8°C
Applications and Recommended Usage?
Quality Tested by Leinco
WB The suggested concentration for this CD1d (clone D5) antibody for use in western blotting is 1-10 μg/ml.
Other Applications Reported In Literature ?
IP
Each investigator should determine their own optimal working dilution for specific applications. See directions on lot specific datasheets, as information may periodically change.

Description

Specificity
Clone D5 recognizes an epitope on human CD1d.
Antigen Distribution
CD1d is expressed on monocytes, DCs, macrophages, and epithelial cells.
Background
CD1d antibody, D5, recognizes human CD1d, also known as CD1.1 and Ly-38, a 48 kDa type 1 membrane glycoprotein that belongs to the cluster of differentiation 1 (CD1) family of glycoproteins. CD1d is expressed on epithelial cells and monocyte-derived antigen presenting cells (APCs), including monocytes, DCs, and macrophages1,2. CD1d is an MHC class I-like molecule involved in the presentation of lipid antigens, such as α-galactosylceramide (α-GalCer), to a highly conserved T cell subset, named invariant natural killer T (iNKT) cells or CD1d-restricted T cells1. Following activation, iNKT cells rapidly proliferate and produce large amounts of cytokines and cytolytic mediators3,4.

Antigen Details

Protein
PubMed
NCBI Gene Bank ID
Research Area
Apoptosis
.
Immunology

References & Citations

1. Van Kaer L., et al. (2004) Nat Rev Immunol. 4(3):231-7
2. Porcelli SA., et al. (2000) Eur J Immunol. 30(12):3468-77
3. Teyton L., et al. (2006) Annu Rev Immunol. 2006;25:297–336
4. Kronenberg M. (2004) Annu Rev Immunol. 23:877–900.
Immunoprecipitation Protocol
General Western Blot Protocol

Formats Available

Products are for research use only. Not for use in diagnostic or therapeutic procedures.