Anti-Mouse CD3 – Purified In vivo GOLD™ Functional Grade

Pricing & Details

Product No.C2443
Clone
17A2
Protein
CD3
Formats AvailableView All
Product Type
Monoclonal Antibody
Alternate Names
T-cell Receptor Complex, CD3ε
Isotype
Rat
IgG2b κ
Applications
CODEX®
,
Costim
,
FC
,
IHC FF
Prod No.
Size
Price
Avail.
Qty
Add to cart
C2443-0.5 mg
0.5 mg
$175.00
In stock
Max:
Min: 1
Step: 1
C2443-1.0 mg
1.0 mg
$355.00
In stock
Max:
Min: 1
Step: 1
C2443-5.0 mg
5.0 mg
$998.00
In stock
Max:
Min: 1
Step: 1

Antibody Details

Product Details

Reactivity Species
Mouse
Host Species
Rat
Immunogen
γ/δ TCR-positive T-T hybridoma D1
Product Concentration
≥ 5.0 mg/ml
Endotoxin Level
≤ 1.0 EU/mg as determined by the LAL method
Purity
≥95% monomer by analytical SEC
>95% by SDS Page
Formulation
This monoclonal antibody is aseptically packaged and formulated in 0.01 M phosphate buffered saline (150 mM NaCl) PBS pH 7.2 - 7.4 with no carrier protein, potassium, calcium or preservatives added.
Product Preparation
Functional grade preclinical antibodies are manufactured in an animal free facility using only In vitro cell culture techniques and are purified by a multi-step process including the use of protein A or G to assure extremely low levels of endotoxins, leachable protein A or aggregates.
Storage and Handling
Functional grade preclinical antibodies may be stored sterile as received at 2-8°C for up to one month. For longer term storage, aseptically aliquot in working volumes without diluting and store at -80°C. Avoid Repeated Freeze Thaw Cycles.
Country of Origin
USA
Applications and Recommended Usage?
Quality Tested by Leinco
FC The suggested concentration for this 17A2 antibody for staining cells in flow cytometry is ≤ 1.0 μg per 106 cells in a volume of 100 μl. Titration of the reagent is recommended for optimal performance for each application.
Other Applications Reported In Literature ?
CODEX®
IHC (Frozen) The suggested concentration for this 17A2 antibody in IHC staining on frozen tissue is 5.0 - 10 μg per ml. Titration of the reagent is recommended for optimal performance for each application.
Each investigator should determine their own optimal working dilution for specific applications. See directions on lot specific datasheets, as information may periodically change.

Description

Specificity
Clone 17A2 recognizes an epitope on mouse CD3.
Antigen Distribution
CD3 is expressed on human peripheral blood lymphocytes, splenic lymphocytes, and the majority of T-CLL and T-ALL cells.
Background
CD3ε is a 20kDa subunit of the TCR complex and is a transmembrane T-cell surface glycoprotein that belongs to the Ig superfamily. It is one of five polypeptide chains that form the TCR complex by associating with the CD3δ, γ and ζ chains, in addition to the TCR α/β or γ/δ chains. CD3 is involved in TCR signaling, enumeration of immunocompetent T-lymphocytes in peripheral blood, and signal transduction during antigen recognition.

Antigen Details

Protein
Ligand/Receptor
Peptide antigen/MHC-complex
Function
Antigen recognition, TCR signal transduction, T cell activation
PubMed
NCBI Gene Bank ID

References & Citations

1. Gallimore, A. et al. (2005) J Immunol. 175(11):7098-102. PubMed
2. Mysliwietz, J. et al. (1992) Blood 80: 2661-2667
Flow Cytometry
IHC

Formats Available

Products are for research use only. Not for use in diagnostic or therapeutic procedures.