Anti-Mouse CD45RB (Clone C363-16A) – PerCP

Home/Primary Monoclonal Antibodies/Surfacetag Mouse CD Markers/Anti-Mouse CD45RB (Clone C363-16A) – PerCP

Pricing & Details

Product No.C1785
Clone
C363-16A
Protein
CD45RB
Formats AvailableView All
Product Type
Monoclonal Antibody
Isotype
Rat
IgG2a
Applications
FC
Prod No.
Size
Price
Avail.
Qty
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C1785-Custom
Custom
In stock
Bulk quantities available. Contact us for pricing.

Antibody Details

Product Details

Reactivity Species
Mouse
Host Species
Rat
Immunogen
Purified Recombinant Mouse CD45RB (>98%)
Product Concentration
0.5 mg/ml
Formulation
This PerCP conjugate is formulated in 0.01 M phosphate buffered saline (PBS) pH 7.4, 150 mM NaCl, 1% BSA and 0.09% sodium azide as a preservative.
Storage and Handling
This PerCP conjugate is stable when stored at 2-8°C. Do not freeze.
Country of Origin
USA
Shipping
Next Day 2-8°C
Applications and Recommended Usage?
Quality Tested by Leinco
FC The suggested concentration for this C363-16A antibody for staining cells in flow cytometry is ≤ 0.25 μg per 106 cells in a volume of 100 μl. Titration of the reagent is recommended for optimal performance for each application.
Additional Reported Applications For Relevant Conjugates ?
IHC (Frozen)
IHC (Paraffin)
IP
For specific conjugates of this clone, review literature for suggested application details.
Each investigator should determine their own optimal working dilution for specific applications. See directions on lot specific datasheets, as information may periodically change.

Description

Specificity
Clone C363-16A recognizes an epitope on mouse CD45RB.
Antigen Distribution
The CD45RB antigen is present on peripheral B-cells, T-cytotoxic cells, at high density on CD4+ T-cells which secrete IL-2 and at low density on CD4+ T-cells which secrete IL-4.1
Background
CD45 is a 180-240kD glycoprotein member of the protein tyrosine phosphatase (PTP) family known for its involvement in regulating a variety of cellular processes including cell growth, differentiation, mitotic cycle, and oncogenic transformation. CD45 and its isoforms are vital regulators of T- and B-cell antigen receptor signaling. CD45 functions through its extracellular domain or through its cytoplasmic domain, and serves as a negative regulator of cytokine receptor signaling via JAK kinase supression. The large extracellular domain is highly glycosylated, and its multiple isoforms allow extensive variation in the structure of its side chains. CD45 isoforms show cell-type and differentiation-stage specific expression that can be used as markers that identify and distinguish between different types of immune cells. CD45R is an isoform of CD45 with a molecular weight of 220 kD. CD45R contains all three possible exons (A, B, and C); making it the longest protein generated from alternative splicing with a migration at 200 kD when isolated from T cells. Furthermore, B cells express CD45R with heavier glycosylation, bringing the molecular weight to 220 kD, hence the name B220. Notably, B220 expression is not only restricted to B cells and may also be expressed on activated T cells, on a subset of dendritic cells, and on other antigen-presenting cells. Additionally, activated and memory T lymphocytes express CD45RO which facilitates T cell activation. CD45RO lacks all three possible exons (A, B, and C), making it the shortest CD45 isoform.

Antigen Details

Protein
Ligand/Receptor
Galectin-1, CD2, CD3, CD4, Thy1
PubMed
NCBI Gene Bank ID
Research Area
Cell Biology
.
Immunology
.
Inhibitory Molecules
.
Neuroscience
.
Neuroscience Cell Markers

References & Citations

1. Bottomly, K. et al. (1989) Eur. J. Immunol. 19:617 2. Johnson, P. et al. (1989) J. Exp. Med. 169:1179 3. Hatchcock, K. S. et al. (1992) J. Immunol. 148:19
Flow Cytometry

Formats Available

Products are for research use only. Not for use in diagnostic or therapeutic procedures.