Anti-Mouse IL-12 – Purified in vivo GOLD™ Functional Grade
Anti-Mouse IL-12 – Purified in vivo GOLD™ Functional Grade
Product No.: I-1175
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Clone C17.8 Target IL-12 Formats AvailableView All Product Type Monoclonal Antibody Alternate Names NKSF, TSF, Maturation Factor, Cytotoxic Lymphocyte Maturation Factor (CLMF), P40 Isotype IgG2a Applications ELISA , ICFC , in vivo , IP , N , WB |
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Antibody DetailsProduct DetailsReactive Species Mouse Host Species Rat Immunogen Purified CHO expressed Recombinant Mouse IL-12 p70 Product Concentration 2.0 mg/ml Endotoxin Level < 1.0 EU/mg as determined by the LAL method Purity ≥95% monomer by analytical SEC ⋅ >95% by SDS Page Formulation This monoclonal antibody is aseptically packaged and formulated in 0.01 M phosphate buffered saline (150 mM NaCl) PBS pH 7.2 - 7.4 with no carrier protein, potassium, calcium or preservatives added. Due to inherent biochemical properties of antibodies, certain products may be prone to precipitation over time. Precipitation may be removed by aseptic centrifugation and/or filtration. Product Preparation Functional grade preclinical antibodies are manufactured in an animal free facility using in vitro cell culture techniques and are purified by a multi-step process including the use of protein A or G to assure extremely low levels of endotoxins, leachable protein A or aggregates. Storage and Handling Functional grade preclinical antibodies may be stored sterile as received at 2-8°C for up to one month. For longer term storage, aseptically aliquot in working volumes without diluting and store at -80°C. Avoid Repeated Freeze Thaw Cycles. Country of Origin USA Shipping Next Day 2-8°C RRIDAB_2737529 Each investigator should determine their own optimal working dilution for specific applications. See directions on lot specific datasheets, as information may periodically change. DescriptionSpecificity Mouse, IL-12/IL-23 p40 subunit (monomer, homodimer and heterodimer IL-12 p35/p40 or IL-23 p19/p40) Background IL-12 plays a role in resistance against pathogens via the differentiation of naive T cells into Th1 cells. It stimulates the growth and function of T cells, blocks formation of new blood vessels, and contributes to antimycobacterial immune response. In addition, it promotes the production of IFN-γ and TNF-α and reduces IL-4 mediated suppression of IFN-γ. Consequently, this enhances the immunostimulatory and immunomodulatory effects of IFN-γ. In addition, there appears to be a link between IL-2 and the signal transduction of IL-12 in NK cells, which enhances the functional response of IL-12 via IFN-γ production and killing of target cells. Furthermore, IL-12 is thought to be associated with autoimmunity. IL-12 was shown to worsen the condition when administered to people already suffering from autoimmune diseases. Comparatively, inhibition of IL-12 (either through IL-12 gene knock-out in mice or treatment of mice with IL-12 mAbs) improved the disease. Antigen DetailsPubMed NCBI Gene Bank ID UniProt.org Research Area Immunology . Innate Immunity . Other Molecules References & Citations1. Kapsenberg, ML. et al. (1997) J. Immunol. 159: 28 2. Ritz, J. et al. (2001) Blood. 97(12):3860-6. 3. Barnes, PF. et al. (1994) J Clin Invest. 93(4):1733-9. Technical Protocols |
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Formats Available
Products are for research use only. Not for use in diagnostic or therapeutic procedures.