Anti-Mouse Ly-6G-BX042 (1A8); Cy5-RX042

Pricing & Details

Product No.L410
Clone
1A8
Protein
Ly-6G
Formats AvailableView All
Product Type
Monoclonal Antibody
Alternate Names
Lymphocyte Antigen 6 Complex
Isotype
Rat
IgG2a
Applications
CODEX®
,
IHC FF
Prod No.
Size
Price
Avail.
Qty
Add to cart
L410-25 Tests
25 tests
$400.00
Pre-order
Max:
Min: 1
Step: 1

Data

CODEX® Details

Barcode
BX042
Reporter
Cy5-RX042
Fluorescent Dye
Cy5 is in the bright red fluorescent dye with an excitation max of 649 nm and an emission max of 666 nm.
Tissue Screened
MCA Sarcoma (Tumor)
Tissue Preparation
FF (Fresh Frozen)

Antibody and Reporter Details

Reactivity Species
Mouse
Host Species
Rat
Formulation
This CODEX® barcoded antibody is formulated in phosphate buffered saline (150 mM NaCl) PBS, EDTA pH 7.2 containing 0.09% sodium azide as a preservative. The CODEX® reporter is lyophilized and formulated in 1X Tris-EDTA (TE) pH 8.0 (10 mM Tris-HCl, 1 mM disodium EDTA, pH 8.0)
Product Preparation
Manufactured in an animal-free facility using only in vitro cell culture techniques, purified by affinity chromatography, and conjugated to a specific barcode under optimal conditions.
State of Matter
CODEX® barcoded antibody - Liquid; CODEX® reporter - Lyophilized
Storage and Handling
This CODEX® barcoded antibody is stable when stored at 2-8°C for up to 1 year (do not freeze). The CODEX® reporter is stable when frozen at -20°C for up to 1 year.
Applications and Recommended Usage?
Quality Tested by Leinco
CODEX®; IHC FF - The suggested dilution for staining tissue in immunohistochemistry - Fresh Frozen (FF) is 1 μl of Anti-Mouse LY-6G (BX042) in a final volume of 200 μl of CODEX® staining buffer.
RRID
AB_2892930
Country of Origin
USA
Each investigator should determine their own optimal working dilution for specific applications. See directions on lot specific datasheets, as information may periodically change.

Description

Specificity
Clone 1A8 recognizes an epitope on mouse Ly6G. Clone 1A8 does not cross react with Ly6C.
Antigen Distribution
Ly6G is expressed by neutrophils.
Background
Ly6G antibody, clone 1A8, recognizes lymphocyte antigen 6 complex locus G6D (Ly6G; also called Gr-1), a 21-25 kDa glycosylphosphatidylinositol (GPI)-anchored protein1. Ly6G belongs to the lymphocyte antigen-6 (Ly6)/urokinase-type plasminogen activator receptor (uPAR) superfamily, characterized by a Ly6/uPAR (LU) domain-containing a three-fingered structural motif stabilized by disulfide bonds2. Ly6G is expressed by murine neutrophils regardless of location and activation1,4,5. Eosinophils may also express low levels of Ly6G5. There is no human ortholog for Ly6G; however, a structurally related L76/uPAR protein, CD177 (also known as HNA-2a, NB1, or PRV-1) is expressed in human neutrophils and is implicated in neutropenia6. Although the exact function and ligand of Ly6G remain unknown, Ly6G ligation may impair neutrophil migration to sites of inflammation via a β2-integrin-dependent mechanism7.

CODEX® reporters are made up of a fluorescent dye and a short oligonucleotide DNA barcode called a CODEX® Tag. Fluorescent reporters enable highly specific detection of corresponding barcodes conjugated to antibodies. The use of spectrally separated dyes allow for precise signal detection in up to three distinct fluorescence channels at one time.

Antigen Details

Protein
PubMed
NCBI Gene Bank ID

References & Citations

1. Fleming TJ, at al. (1993) J Immunol. 151(5):2399-408
2. Tsetlin VI. (2015) Trends Pharmacol Sci. 36(2):109-23
3. Daley JM, et al. (2008) J Leukoc Biol. 83(1):64-70
4. Lee PY, et al. (2013) J Leukoc Biol. 94(4):585-594
5. Percopo CM, et al. (2017) J Leukoc Biol. 101(1):321-328.
6. Stroncek DF. (2007) Curr Opin Hematol. 14(6):688-93
7. Wang JX, et al. (2012) Blood. 120(7):1489-1498
IHC

Formats Available

Products are for research use only. Not for use in diagnostic or therapeutic procedures.
Cookie Policy
Leinco Technologies uses cookies to improve your experience and our website service. By continuing to browse our website, you accept our cookie policy.